https://github.com/alexanderlabwhoi/areyouok-mer

Directory for the Snakemake workflow for checking sourmash similarities of protists

https://github.com/alexanderlabwhoi/areyouok-mer

Science Score: 13.0%

This score indicates how likely this project is to be science-related based on various indicators:

  • CITATION.cff file
  • codemeta.json file
  • .zenodo.json file
  • DOI references
    Found 1 DOI reference(s) in README
  • Academic publication links
  • Academic email domains
  • Institutional organization owner
  • JOSS paper metadata
  • Scientific vocabulary similarity
    Low similarity (3.5%) to scientific vocabulary
Last synced: 6 months ago · JSON representation

Repository

Directory for the Snakemake workflow for checking sourmash similarities of protists

Basic Info
  • Host: GitHub
  • Owner: AlexanderLabWHOI
  • License: mit
  • Language: Jupyter Notebook
  • Default Branch: master
  • Homepage:
  • Size: 10.3 MB
Statistics
  • Stars: 0
  • Watchers: 2
  • Forks: 0
  • Open Issues: 1
  • Releases: 0
Created about 6 years ago · Last pushed almost 6 years ago

https://github.com/AlexanderLabWHOI/areyouok-mer/blob/master/

# areyouok-mer
eukhashing project!

Directory for the Snakemake workflow for checking sourmash similarities of _Thaps_ experimental sequences and assembled genomes

## Steps

Branch 1: Raw Sequences (experiments pulled from online)
1. Interleave/concatenate
2. Do error trimming (coverage 5-10%) - good tool available on Conda = https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-017-1469-3
https://anaconda.org/bioconda/afterqc
3. Sourmash with k = 21, 31, 51, track abundance 

Branch 2: pre-assembled
1. Sourmash step 
2. Run on whole genome as well as just the coding region of the genome
3. Just work with cleaned assemblies, not combined assembles

Owner

  • Name: Alexander Lab @ WHOI
  • Login: AlexanderLabWHOI
  • Kind: organization
  • Email: halexander@whoi.edu
  • Location: Woods Hole, MA

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