https://github.com/asadprodhan/bioinformatics_core

Bioinf Tutorials

https://github.com/asadprodhan/bioinformatics_core

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bash-scripting bioinformatics bioinformatics-pipeline bioinformatics-scripts coding r
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Bioinf Tutorials

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  • Owner: asadprodhan
  • License: gpl-3.0
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bash-scripting bioinformatics bioinformatics-pipeline bioinformatics-scripts coding r
Created over 2 years ago · Last pushed 6 months ago
Metadata Files
Readme License

README.md

Bioinformatics Core

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CONTENT

Chapter 01. Bioinformatics Basics

Chapter 02. An Introduction to R Programming

Chapter 03. An Introduction to Bash Commands

Chapter 04. An Introduction to Remote Computing

Chapter 05. How to Retrieve Public Data

Chapter 06. DNA Data Wrangling

Chapter 07. Nanopore Sequencing Data Analysis

Chapter 08. Bioinformatics Tools for Pest and Pathogen Diagnosis

Chapter 09. Bioinformatics Workflow for Transcriptome Analysis Using Illumina Sequencing

Chapter 10. Bioinformatics Workflow for Microbiome Data Analysis

Chapter 11. Writing Manuscripts

Chapter 12. My Bioinformatics Presentations


CHAPTER 01. BIOINFORMATICS BASICS

01. How to set up Windows Subsystem for Linux (WSL)?

02. Navigating a Linux Computer

03. Getting started with bioinformatics coding

04. A beginner's guide to Bioinformatics

05. About the PATH


CHAPTER 02. R

01. Why graduate students should learn R?

02. Setting up RStudio

03. An Introduction to R Coding

04. Data visualisation and analysis in R


CHAPTER 03. BASH

01. Navigating a Linux Computer

02. Command Line Basics

03. Bash Script to Extract Individual Entries from a Large fasta File

04. Bash Scrip To Show Directory Properties


CHAPTER 04. REMOTE COMPUTING

01. How to Connect to a Remote Linux Computer

02. How to Connect to Pawsey’s Nimbus Instance

03. How to Mount Cloud NAS Device to Linux Computer


CHAPTER 05. RETRIEVE PUBLIC DATA

01. Genome sequence metadata [manual]

02. Genome sequence metadata [command line]

03. Genome sequences [manual]

04. Genome sequences using datasets [command line]

05. Genome sequences using wget [command line]

06. RefSeq genome sequences using wget [command line]

07. Downloading genomes using the accession number

08. Downloading genomes from RefSeq

09. Downloading raw reads


CHAPTER 06. DNA DATA WRANGLING

01. How to Filter Out Contigs from a Genome Assembly

02. How to Extract Individual Entries from a Large fasta File

03. Hoq to Filtering out Invalid Characters from fastq Reads

04. How to Extract Individual Entries from a Large fasta File

05. How to Automate Gene Seq Extraction from Multiple Genomes

06. How to Split a Concatenated fasta File into Individual Files


CHAPTER 07. NANOPORE

01. An Introduction to Nanopore DNA Sequencing and Data Analysis

02. Automating Sample Labelling after Nanopore Sequencing

03. GPU-Accelerated Guppy Basecalling

04. Dorado Basecalling

05. Dorado Basecalled Reads Improve Old Bacterial Genome Assemblies

06. How to Channel Sequencing Reads from Multiple Subdirectories into Nextflow Pipeline

07. Miniasm (setting up and running miniasm assembler

08. Reducing Storage Space by Converting Nanopore fast5 Files to slow5


CHAPTER 08. PEST AND PATHOGEN DIAGNOSIS

01. Blastn (database download, execution, hit sequence extraction, and possible errors)

02. ANI (average nucleotide identity)

03. Bacterial ID (phylogenetic tree reconstruction using UBCG pipeline)

04. Kraken 2 (running on HPC using singularity container and nextflow?)

05. sangerFlow (a Sanger sequencing-based bioinformatics pipeline for pests and pathogens identification)

06. phyloTree (an automated, reproducible, and scalable workflow for Bayesian phylogenetic analysis using Nextflow and Singularity)

07. phyloTreeHPC (an automated, reproducible, and scalable workflow for Bayesian phylogenetic analysis using a HPC cluster, Nextflow, and Singularity container)

08. Diagnosing Bacteria Using Illumina Sequencing


CHAPTER 09. TRANSCRIPTOMICS

01. How to Analysis Transcriptome Illumina Sequencing Reads

02. How to Make Hierarchical Clustering Heatmap of the Target DEGs and Plot Individual Clusters

03. How to Do GO Term Enrichment Analysis using GOMCL


CHAPTER 10. MICROBIOME

01. Environmental Rhizosheath Microbiome Sampling Protocol

02. A Bioinformatics Pipeline for 16s Microbiome Data


CHAPTER 11. BUILDING CUSTOMISED DATABASE

01. How to Build a Customised Kraken2 Database

02. How to Build a Customised Bracken Database


CHAPTER 12. WRITING

01. Writing and Publishing Protocols using GitHub

02. Publishing a paper. Professor Hans Lambers


CHAPTER 13. PRESENTATIONS

01. An introduction to Nanopore DNA sequencing and data analysis. Nanopore Workshop for Biosecurity, Curtin University, Perth, Australia, 27-29 June 2023.

02. Preparing for a career after university. Career Development Workshop, UWA Society of Bangladeshi Culture (USBC), The University of Western Australia, Perth, Australia, 26 August 2023.

03. Command Line Basics. Nanopore Workshop for Biosecurity, Curtin University, Perth, Australia, 27-29 June 2023.

04. Introductory Bioinformatics: Where do I start? Murdoch Bioinformatics, Murdoch University, Perth, Australia, 04 April 2024.


Owner

Laboratory Scientist at DPIRD. My work involves Oxford Nanopore Sequencing and Bioinformatics for pest and pathogen diagnosis.

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