cellenium

Cellenium is a FAIR and scalable interactive visual analytics app for scRNA-Seq data. It allows to: * organize and semantically find scRNA studies with ontologized metadata for tissues and diseases * explore cell types and other cell annotations in UMAP space * find differentially expressed genes based on clusters of annotated cells * view the expression of a single gene (or a few selected genes) in the UMAP plot or as grouped violin plots * draw coexpression plots for pairs of genes, explore the cell types contained in the plots * add new cell annotations based on plot selections, see differentially expressed genes for a selected group of cells * find genes which expression is highly correlated to a query gene * find marker genes in all imported studies and qualitatively compare gene expression across studies

Link to publication: https://doi.org/10.1093/bioinformatics/btad349

Link to showcase: https://youtu.be/U71qIK-Mqlc

System Overview

Cellenium imports scRNA expression data and cell annotations in H5AD format. We provide jupyter notebooks for downloading some publicly available scRNA studies, normalize the data if necessary, and calculate differentially expressed genes, a UMAP projection and other study data that is needed for Cellenium's features to work.

Cellenium is a web application that accesses a PostgreSQL database via GraphQL API. Some API features, like server-side rendered plots, depend on Python stored procedures. The graphqlapiusage folder contains a couple of example queries to illustrate the API capabilities.

The setup steps below automate the download and creation of appropriate H5AD files, docker image build, database schema setup and data ingestion.

Setting up

Preparation of CellO data files (workaround for https://github.com/deweylab/CellO/issues/29 ):

bash mkdir scratch/cello_resources curl https://deweylab.biostat.wisc.edu/cell_type_classification/resources_v2.0.0.tar.gz >scratch/cello_resources/resources_v2.0.0.tar.gz tar -C scratch/cello_resources -zxf scratch/cello_resources/resources_v2.0.0.tar.gz

Cellenium setup, including execution of study data processing notebooks (initially, this will take a couple of hours to run).

```bash

builds docker images and runs the whole stack

until you run the "make reset_database" step below, error messages about the missing "postgraphile" user pile up... you can ignore them for now.

docker compose up conda env create -f dataimport/environment.yml conda activate celleniumimport

'test_studydata' should contain data to cover all application features, but is small enough to be imported in a few minutes

make resetdatabase teststudydata_import

'normal_studydata': real life studies (i.e. with full amount of cells and genes)

make normalstudydataimport

we have one for atac

make atacstudydataimport

and one for cite

make citestudydataimport ```

The GraphQL API explorer is available at http://localhost:5000/postgraphile/graphiql . Postgraphile will listen to changes in the database schema and the updated API is visible immediately.

The cellenium webapp 'production build' static site is hosted in the 'client' container, see http://localhost:6002/ . For development, you run (cd client && yarn && yarn start) to install the webapp's dependencies and have a hot-reloaded webapp.

Before you process and import the huge example study (there are two additional make targets for that), edit the beginning of heart_failure_reichart2022*.ipynb and define the download URL as described in the notebooks.

manually executing the study data preparation jupyter notebooks

The notebooks are run in headless mode by make. To create new notebooks and explore datasets:

bash (cd data_import && PYTHONPATH=$(pwd) jupyter-lab)