Science Score: 57.0%

This score indicates how likely this project is to be science-related based on various indicators:

  • CITATION.cff file
    Found CITATION.cff file
  • codemeta.json file
    Found codemeta.json file
  • .zenodo.json file
    Found .zenodo.json file
  • DOI references
    Found 4 DOI reference(s) in README
  • Academic publication links
  • Academic email domains
  • Institutional organization owner
  • JOSS paper metadata
  • Scientific vocabulary similarity
    Low similarity (4.0%) to scientific vocabulary
Last synced: 6 months ago · JSON representation ·

Repository

Basic Info
  • Host: GitHub
  • Owner: Satovase
  • Language: R
  • Default Branch: main
  • Size: 51.8 KB
Statistics
  • Stars: 0
  • Watchers: 1
  • Forks: 0
  • Open Issues: 0
  • Releases: 5
Created almost 2 years ago · Last pushed over 1 year ago
Metadata Files
Readme Citation

README.md

paper01_emu-wing

This repository contains R codes used to perform analysis in Tsuboi, E., Ono, S.F., Cordeiro, I.R. et al. Immobilization secondary to cell death of muscle precursors with a dual transcriptional signature contributes to the emu wing skeletal pattern. Nat Commun 15, 8153 (2024). https://doi.org/10.1038/s41467-024-52203-x

Raw single-cell transcriptome sequencing data (FASTQ) of emu is available in the DNA Data Bank of Japan (Accession numbers: DRA014432 and DRA017391; Bioproject: PRJDB16987 and PRJDB13845).

Raw single-cell transcriptome sequencing data (FASTQ) of stage 24 (SRA accession numbers: SRR14570167 to SRR14570174) and stage 27 (SRA accession numbers: SRR14570175 to SRR14570178) were downloaded from the Sequence Read Archive (SRA). These data were previously published in Feregrino C, Tschopp P. Assessing evolutionary and developmental transcriptome dynamics in homologous cell types. Dev Dyn. 2022 Sep;251(9):1472-1489. https://doi.org/10.1002/dvdy.384

Preprocessing using cellranger

Emumakecellranger_count

Chickenmakecellranger_count

Creating figures using Seurat

Emu_stage20-21.R: Figure 3a, 3b, S9, S10, Supplymentary table 1

Emu_stage25.R: Figure 3d, 3e, 3g, 5a, S11, S12, Supplymentary table 2, Supplymentary table 3

Chicken_stage24.R: Figure S13a, S13b, S13d, Supplymentary table 4

Chicken_stage27.R: Figure S13e, S13f, S13h, Supplymentary table 5

License

This project is covered under the Apache 2.0 License.

Owner

  • Login: Satovase
  • Kind: user

Citation (CITATION.cff)

cff-version: 1.1.0
message: "If you use this software, please cite it as below."
authors:
- family-names: Ono
  given-names: Satomi
orcid: https://orcid.org/0009-0003-4386-3447
title: Immobilization secondary to cell death of muscle precursors with a dual transcriptional signature contributes to the emu wing skeletal pattern
version: 7
date-released: 2024-09-17
        

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