https://github.com/biop/multiview-reconstruction

Software for the reconstruction of multi-view microscopic acquisitions like Selective Plane Illumination Microscopy (SPIM) Data

https://github.com/biop/multiview-reconstruction

Repository

Software for the reconstruction of multi-view microscopic acquisitions like Selective Plane Illumination Microscopy (SPIM) Data

https://github.com/BIOP/multiview-reconstruction/blob/master/

[![](https://github.com/PreibischLab/multiview-reconstruction/actions/workflows/build-main.yml/badge.svg)](https://github.com/PreibischLab/multiview-reconstruction/actions/workflows/build-main.yml)

#  Introduction & Overview

Selective Plane Illumination Microscopy
([SPIM, Science, 305(5686):1007-9](http://www.sciencemag.org/content/305/5686/1007))
allows in toto imaging of large specimens by acquiring image stacks from
multiple angles. However, to realize the full potential of these acquisitions
the data needs to be reconstructed. This project implements several algorithms
for the registration and fusion of multi-angle SPIM acquisitions.

# Installation

The easiest is to get the SPIM Reconstruction as part of [Fiji](http://fiji.sc/).

See also the [SPIM Registration page on the ImageJ
wiki](http://imagej.net/Multiview-Reconstruction).

For questions, bug reports, remarks and comments just use github here or send
me an email: stephan.preibisch@einstein.yu.edu

# Citation

Please note that the SPIM reconstruction plugin available through Fiji, is
based on publications. If you use it successfully for your research please be
so kind to cite our work:

* S. Preibisch, S. Saalfeld, J. Schindelin and P. Tomancak (2010) "Software for
  bead-based registration of selective plane illumination microscopy data",
  Nature Methods, 7(6):418-419.
  [Webpage](http://www.nature.com/nmeth/journal/v7/n6/full/nmeth0610-418.html)

* S. Preibisch, F. Amat, E. Stamataki, M. Sarov, R.H. Singer, E. Myers and P.
  Tomancak (2014) Efficient Bayesian-based Multiview Deconvolution, Nature
  Methods, 11(6):645-648.
  [Webpage](http://www.nature.com/nmeth/journal/v11/n6/full/nmeth.2929.html)

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