https://github.com/bmascat/lfq-proteomics-pipeline

All code, bash scrypts and pipelines used to analyse and visualise proteomic data from my experiments with my first Crispr KO model.

https://github.com/bmascat/lfq-proteomics-pipeline

Science Score: 26.0%

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Keywords

bioinformatics goterm over-representation-analysis proteomics proteomics-data-analysis
Last synced: 6 months ago · JSON representation

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All code, bash scrypts and pipelines used to analyse and visualise proteomic data from my experiments with my first Crispr KO model.

Basic Info
  • Host: GitHub
  • Owner: bmascat
  • License: mit
  • Language: R
  • Default Branch: master
  • Homepage:
  • Size: 42 KB
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bioinformatics goterm over-representation-analysis proteomics proteomics-data-analysis
Created over 5 years ago · Last pushed over 3 years ago
Metadata Files
Readme License

README.md

Proteomic analysis of CRISPR Hela KO Alms1 after TGF-B stimulation

All code and pipelines used to analyze and visualize proteomic data from my experiments with my first Crispr KO model

image

Differenticial expression analysis was performed with DEP package:

Zhang X, Smits A, van Tilburg G, Ovaa H, Huber W, Vermeulen M (2018). “Proteome-wide identification of ubiquitin interactions using UbIA-MS.” Nature Protocols, 13, 530–550.

Go-Terms analysis and data results visualizations was performed with Clusterprofiler and GOplot packages to R:

Yu G, Wang L, Han Y, He Q (2012). “clusterProfiler: an R package for comparing biological themes among gene clusters.” OMICS: A Journal of Integrative Biology, 16(5), 284-287. doi: 10.1089/omi.2011.0118.

Walter, Wencke, Fátima Sánchez-Cabo, and Mercedes Ricote. "GOplot: an R package for visually combining expression data with functional analysis." Bioinformatics (2015): btv300.

Owner

  • Name: Brais
  • Login: bmascat
  • Kind: user

Data science, bioinformatics and software development

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