piezo_copulation_source_code

"Piezo-mediated mechanosensation contributes to stabilizing copulation posture and reproductive success in Drosophila males"

https://github.com/kamikouchi-lab/piezo_copulation_source_code

Science Score: 36.0%

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"Piezo-mediated mechanosensation contributes to stabilizing copulation posture and reproductive success in Drosophila males"

Basic Info
  • Host: GitHub
  • Owner: Kamikouchi-lab
  • License: mit
  • Language: Jupyter Notebook
  • Default Branch: main
  • Size: 3.36 GB
Statistics
  • Stars: 1
  • Watchers: 1
  • Forks: 0
  • Open Issues: 0
  • Releases: 1
Created over 3 years ago · Last pushed almost 3 years ago
Metadata Files
Readme License Citation

README.md

Piezo-mediated mechanosensation contributes to stabilizing copulation posture and reproductive success in Drosophila males

DOI

Figure 2C, D

  • DeepLabCut model for tracking the flies position : malecopulationposture_model
  • Calculating the male copulation posture : reshape_dataset.ipynb
  • Plotting and statistics analysis : piezoratioand_bunsan.R

Figure 2E

  • DeepLabCut model for tracking the flies position : malecopulationposture_model
  • Calculating the male copulation posture and plotting : reshape_dataset.ipynb

Figure 3A

  • Plotting and statistics analysis : cop_ratio.R

Figure 3B

  • Plotting and statistics analysis : cop_ratio.R

Figure 3C

  • Plotting and statistics analysis : cop_ratio.R

Figure 5C, H

  • Plotting and statistics analysis : copulation_opt.R

Figure 5F

  • DeepLabCut model for tracking the flies position : malecopulationposturemovementmodel
  • Calculating the male copulation position : laseroptanalysis.ipynb
  • Plotting and statistics analysis : laser_analysis.R

Figure 5G

  • DeepLabCut model for tracking the flies position : malecopulationposturemovementmodel
  • Calculating the male copulation position : laseroptanalysis.ipynb
  • Plotting and statistics analysis : laser_analysis.R

Figure S2A-K

  • DeepLabCut model for tracking the flies position : malecopulationposture_model
  • Calculating the male copulation posture : reshapedataset20-30.ipynb
  • Plotting and statistics : ratiographdrawing.R

Figure S3A

  • Plotting and statistics : kickcopulationanalysis.R

Figure S3B

  • Plotting and statistics : genitaltouchcount_analysis.R

Figure S4A

  • Plotting and statistics : activity_analysis.R

Figure S4B

  • Plotting and statistics : flyscaleanalysis.R

Figure S5

  • Plotting and statistics : copulationmixingassay.R

Figure S7A, B

  • Plotting and statistics : copulationopthead_grooming.R

Figure S9B

  • Calculating the male copulation posture : laser_map.ipynb
  • Plotting and statistics :laserstrengthanalysis.R

Figure S14B

  • DeepLabCut model for tracking the flies position : malecopulationposture_model
  • Calculating the DLC time : reshape_dataset.ipynb
  • Plotting and statistics : piezoratioand_bunsan.R

Closed-optogenetic system for optogenetic assays with LED

  • DeepLabCut model : copulationdetectionmodelforLED
  • Bonsai script : copulationdetectLED.bonsai
  • Arduino script : CreatePWMstimubonsaitoarduino_LED.ino, StandardFirmata.ino

Closed-optogenetic system for optogenetic assays with a laser

  • DeepLabCut model : copulationdetectionmodelforlaser
  • Bonsai script : copulationdetectlaser.bonsai
  • Arduino script : CreatePWMstimubonsaitoarduino_laser.ino, StandardFirmata.ino

Owner

  • Name: Kamikouchi-Lab
  • Login: Kamikouchi-lab
  • Kind: organization

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