https://github.com/bodenmillergroup/saber-imc_publication

https://github.com/bodenmillergroup/saber-imc_publication

Science Score: 13.0%

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Last synced: 9 months ago · JSON representation

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  • Host: GitHub
  • Owner: BodenmillerGroup
  • License: mit
  • Default Branch: main
  • Size: 24.4 KB
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Created almost 4 years ago · Last pushed about 3 years ago
Metadata Files
Readme License

README.md

SABER-IMC_publication

List of Cellprofiler pipelines and R codes used for the analysis of the data used for the paper "DNA-barcoded signal amplification for imaging mass cytometry enables sensitive and highly multiplexed tissue imaging" (Hosogane et al.), about signal amplification for imaging mass cytometry.

Signal and background intensity quantification

thresholding_dev1.cppipe: - Create signal and background region masks by thresholding using CellProfiler.

Single cell segmentation

calculate_intensity.cppipe: - Quantification of mean signal and background intensities from region masks (Fig.3, Fig.S2, Fig.S3)

SABER_preprocessing script

SABER_preprocessing.Rmd : - Conversion of single cell data from CellProfiler to SingleCellExperiment object. - Data scaling and filtering

SABER_celltyping scripts

SABER_celltyping.Rmd : - Single cell phenotyping and expression quantification (Fig.5,S5) - Visualisation of cell populations and markers (Fig.5,S5)

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  • Name: BodenmillerGroup
  • Login: BodenmillerGroup
  • Kind: organization

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