https://github.com/brickmanlab/proks-et-al-2023

https://github.com/brickmanlab/proks-et-al-2023

Science Score: 36.0%

This score indicates how likely this project is to be science-related based on various indicators:

  • CITATION.cff file
  • codemeta.json file
    Found codemeta.json file
  • .zenodo.json file
  • DOI references
    Found 3 DOI reference(s) in README
  • Academic publication links
    Links to: zenodo.org
  • Academic email domains
  • Institutional organization owner
  • JOSS paper metadata
  • Scientific vocabulary similarity
    Low similarity (3.8%) to scientific vocabulary
Last synced: 10 months ago · JSON representation

Repository

Basic Info
  • Host: GitHub
  • Owner: brickmanlab
  • Language: Jupyter Notebook
  • Default Branch: master
  • Size: 11.5 MB
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  • Watchers: 1
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Created about 3 years ago · Last pushed over 1 year ago
Metadata Files
Readme

README.md

proksetal_2023

Complemetary analysis to nf-core/marsseq.

Dataset available at DOI

nf-core/marsseq

```bash module load java/11.0.15 nextflow/22.10.6 singularity/3.8.0

sh nf-coretower.sh mm10ref nextflow run $PIPELINEPATH/nf-core-marsseq \ --input $DATAPATH/SB26/design.csv \ --buildreferences \ --genomesbase /scratch/Brickman/references \ --genome mm10

sh nf-coretower.sh SB26 nextflow run $PIPELINEPATH/nf-core-marsseq \ --input $DATAPATH/SB26/design.csv \ --genomesbase /scratch/Brickman/references \ --genome mm10

sh nf-coretower.sh SB28 nextflow run $PIPELINEPATHnf-core-marsseq \ --input $DATAPATH/SB28/design.csv \ --genomesbase /scratch/Brickman/references \ --genome mm10 ```

MARS-seq2.0

```bash module load miniconda/latest

source activate ngs cookiecutter https://github.com/brickmanlab/mars-seq2-inhouse

source activate mars python helpers/prepare.py --input $PIPELINEPATH/pipeline/SB26/ --output testdata

split cells per Batch

scripts/splitfastqs.sh testdata/rawreads/SB26/origfiles/ testdata/rawreads/SB26/ 4000000 scripts/splitfastqs.sh testdata/rawreads/SB28/origfiles/ testdata/rawreads/SB28/ 4000000

run pipeline

scripts/runpipelinelocally.sh test_data ```

Owner

  • Name: Brickman group
  • Login: brickmanlab
  • Kind: organization
  • Location: Copenhagen

Professor Joshua Brickman at Center for Stem Cell Medicine (reNEW), University of Copenhagen

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