scp-ingest-pipeline

File Ingest Pipeline for Single Cell Portal

The SCP Ingest Pipeline is an ETL pipeline for single-cell RNA-seq data.

Prerequisites

• Python 3.10
• Google Cloud Platform project
• Suitable service account (SA) and MongoDB VM in GCP. SA needs roles "Editor", "Genomics Pipelines Runner", and "Storage Object Admin". Broad Institute engineers: see instructions here.
• SAMtools, if using ingest/make_toy_data.py
• Tabix, if using ingest/genomes/genomes_pipeline.py

Install

Native

Fetch the code, boot your virtualenv, install dependencies:

git clone git@github.com:broadinstitute/scp-ingest-pipeline.git cd scp-ingest-pipeline python3 -m venv env --copies source env/bin/activate pip install -r requirements.txt source scripts/setup-mongo-dev.sh

Docker

With Docker running and gcloud authenticated on your local machine, run:

scripts/docker-compose-setup.sh

If on Apple silicon Mac (e.g. M1), and performance seems poor, consider generating a docker image using the arm64 base. Example test image: gcr.io/broad-singlecellportal-staging/single-cell-portal:development-2.2.0-arm64, usage:

scripts/docker-compose-setup.sh -i development-2.2.0-arm64

To update dependencies when in Docker, you can pip install from within the Docker Bash shell after adjusting your requirements.txt. If you close your shell after that, your newly installed dependencies will be lost. Dependencies only persist after merging your new requirements.txt into development. TODO (SCP-4941): Add entry-point script to run pip install.

Optional

To use ingest/make_toy_data.py:

brew install samtools

To use ingest/genomes/genomes_pipeline.py:

brew install tabix

Git hooks

After installing Ingest Pipeline, add Git hooks to help ensure code quality:

pre-commit install && pre-commit install -t pre-push

The hooks will expect that git-secrets has been set up. If you are a Broad Institute employee who has not done this yet, please see: broadinstitute/singlecellportal_configs for specific guidance.

Bypass hooks

In rare cases, you might need to skip Git hooks, like so:

• Skip commit hooks: git commit ... --no-verify
• Skip pre-push hooks: git push ... --no-verify

Test

After installing:

``` source env/bin/activate cd tests

Run all tests

pytest ```

Some common pytest usage examples (run in /tests):

```

Run all tests and see print() output

pytest -s

Run only tests in test_ingest.py

pytest test_ingest.py

Run all tests, show code coverage metrics

pytest --cov=../ingest/ ```

For more, see https://docs.pytest.org/en/stable/usage.html.

Testing in Docker locally

If you have difficulties installing and configuring scp-ingest-pipeline due to hardware issues (e.g. Mac M1 chips), you can alternatively test locally by building the Docker image and then running any commands inside the container. There are some extra steps required, but this sidesteps the need to install packages locally.

1. Build the image

Run the following command to build the testing Docker image locally (make sure Docker is running first). This build command will incorporate any changes in the local instance of your repo, committed or not:

docker build -t ingest-pipeline:test-candidate .

Note - the base Ubuntu image used in the Dockerfile comes from Google Container Registry (GCR, aka gcr.io), if this is your first time doing docker build you may need to configure Docker to use the Google Cloud CLI to authenticate requests to Container Registry:

gcloud auth configure-docker

Pro-Tip: For local builds, you can try adding docker build options --progress=plain (for more verbose build info) and/or --no-cache (when you want to ensure a build with NO cached layers)

2. Set up environment variables

Run the following to pull database-specific secrets out of Google Secrets Manager (GSM):

source scripts/setup-mongo-dev.sh

Now run env to make sure you've set the following values:

MONGODB_USERNAME=single_cell DATABASE_NAME=single_cell_portal_development MONGODB_PASSWORD=<password> DATABASE_HOST=<ip address>

3. Print out your service account keyfile

Run the following to export out your default service account JSON keyfile:

GOOGLE_PROJECT=$(gcloud info --format="value(config.project)") gcloud secrets versions access latest --project=$GOOGLE_PROJECT --secret=default-sa-keyfile | jq > /tmp/keyfile.json

4. Start the Docker container

Run the container, passing in the proper environment variables:

docker run --name scp-ingest-test -e MONGODB_USERNAME="$MONGODB_USERNAME" -e DATABASE_NAME="$DATABASE_NAME" \ -e MONGODB_PASSWORD="$MONGODB_PASSWORD" -e DATABASE_HOST="$DATABASE_HOST" \ -e GOOGLE_APPLICATION_CREDENTIALS=/tmp/keyfile.json --rm -it \ ingest-pipeline:test-candidate bash

Note: on an M1 machine, you may see this message:

WARNING: The requested image's platform (linux/amd64) does not match the detected host platform (linux/arm64/v8) and no specific platform was requested

5. Copy keyfile to running container

In a separate terminal window, copy the JSON keyfile from above to the expected location:

docker cp /tmp/keyfile.json scp-ingest-test:/tmp

You can now run any ingest_pipeline.py command you wish inside the container.

Use

Run this every time you start a new terminal to work on this project:

source env/bin/activate

See ingest_pipeline.py for usage examples.

Troubleshooting during set up

If you run into an error like: "... [SSL: CERTIFICATEVERIFYFAILED] certificate verify failed ... " try:

• Open terminal
• cd to where python is installed
• Run the certificates command with /Applications/Python\ < Your Version of Python Here >/Install\ Certificates.command

If you run into an error like "ModuleNotFoundError: No module named 'google'" try:

• Open terminal
• Run pip install --upgrade google-api-python-client