https://github.com/broadinstitute/deepometry

Image classification for imaging flow cytometry.

https://github.com/broadinstitute/deepometry

Science Score: 33.0%

This score indicates how likely this project is to be science-related based on various indicators:

  • CITATION.cff file
  • codemeta.json file
  • .zenodo.json file
  • DOI references
    Found 2 DOI reference(s) in README
  • Academic publication links
    Links to: pubmed.ncbi, ncbi.nlm.nih.gov
  • Committers with academic emails
    1 of 5 committers (20.0%) from academic institutions
  • Institutional organization owner
  • JOSS paper metadata
  • Scientific vocabulary similarity
    Low similarity (11.2%) to scientific vocabulary

Keywords from Contributors

microscopy profiling
Last synced: 10 months ago · JSON representation

Repository

Image classification for imaging flow cytometry.

Basic Info
Statistics
  • Stars: 27
  • Watchers: 15
  • Forks: 7
  • Open Issues: 6
  • Releases: 0
Created about 9 years ago · Last pushed over 1 year ago
Metadata Files
Readme License

README.md

Deepometry

Deep learning-based image classification and featurization for imaging (flow) cytometry.

This workflow was originally built for imaging flow cytometry data but can be readily adapted for microscopic images of isolated single objects. The modified implementation of ResNet50 allows researchers to use any image frame size and any number of color channels.

Installation

A full installation guide can be found here. Briefly, the following dependencies are needed: - Python 3.6 - Tensorflow-gpu 1.9.0 - Keras 2.1.5 - Numpy 1.18.1 - Scipy 1.4.1 - Keras-resnet 0.0.7 - Java JDK 8.0 or 11.0 - Python-bioformats 1.5.2

Once the above dependencies are installed, clone this Deepometry repository by :

git clone https://github.com/broadinstitute/deepometry.git
cd deepometry
pip install .

If you want to install deepometry in development mode, run:

pip install --editable .[development]

Use

Execute Deepometry functions through any of the following interfaces:

CLI

Switch to CLI branch:

git checkout CLI

Display a list of available subcommands:

deepometry --help

To display subcommand use and options:

deepometry SUBCOMMAND --help

IPYNB

Switch to IPYNB branch:

git checkout IPYNB

Use these Jupyter notebooks.

GUI (recommended)

Switch to GUI branch:

git checkout GUI

python Deepometry_GUI.py

Open a web-browser, navigate to http://127.0.0.1:5000/ or http://localhost:5000/

Full view GUI

Publications

Doan M, Sebastian JA, Caicedo JC, et al. Objective assessment of stored blood quality by deep learning. Proc Natl Acad Sci U S A. 2020;117(35):21381-21390. doi:10.1073/pnas.2001227117

Doan M, Case M, Masic D, et al. Label-Free Leukemia Monitoring by Computer Vision. Cytometry A. 2020;97(4):407-414. doi:10.1002/cyto.a.23987

Owner

  • Name: Broad Institute
  • Login: broadinstitute
  • Kind: organization
  • Location: Cambridge, MA

Broad Institute of MIT and Harvard

GitHub Events

Total
  • Watch event: 1
  • Delete event: 1
  • Push event: 1
  • Pull request event: 2
  • Create event: 1
Last Year
  • Watch event: 1
  • Delete event: 1
  • Push event: 1
  • Pull request event: 2
  • Create event: 1

Committers

Last synced: over 2 years ago

All Time
  • Total Commits: 65
  • Total Committers: 5
  • Avg Commits per committer: 13.0
  • Development Distribution Score (DDS): 0.477
Past Year
  • Commits: 0
  • Committers: 0
  • Avg Commits per committer: 0.0
  • Development Distribution Score (DDS): 0.0
Top Committers
Name Email Commits
mcquin m****n@b****g 34
mcquin m****l@g****m 17
minh-doan v****n@g****m 8
Minh Doan d****y@g****m 3
Claire McQuin m****n 3
Committer Domains (Top 20 + Academic)