turner-lab-to-nwb

NWB conversion scripts for Turner lab data to the Neurodata Without Borders data format.

Installation

The package can be installed directly from GitHub, which has the advantage that the source code can be modified if you need to amend some of the code we originally provided to adapt to future experimental differences. To install the conversion from GitHub you will need to use git (installation instructions). We also recommend the installation of conda (installation instructions) as it contains all the required machinery in a single and simple install.

From a terminal (note that conda should install one in your system) you can do the following:

git clone https://github.com/catalystneuro/turner-lab-to-nwb cd turner-lab-to-nwb conda env create --file make_env.yml conda activate turner_lab_to_nwb_env

This creates a conda environment which isolates the conversion code from your system libraries. We recommend that you run all your conversion related tasks and analysis from the created environment in order to minimize issues related to package dependencies.

Alternatively, if you want to avoid conda altogether (for example if you use another virtual environment tool) you can install the repository with the following commands using only pip:

git clone https://github.com/catalystneuro/turner-lab-to-nwb cd turner-lab-to-nwb pip install -e .

Note: both of the methods above install the repository in editable mode.

Repository structure

Each conversion is organized in a directory of its own in the src directory:

turner-lab-to-nwb/
├── LICENSE
├── make_env.yml
├── pyproject.toml
├── README.md
├── requirements.txt
├── setup.py
└── src
    ├── turner_lab_to_nwb
    │   ├── asap_embargo
    │   └── asap_tdt
    │       ├── extractors
    │       │   ├── __init__.py
    │       │   ├── asap_tdt_filtered_recordingextractor.py
    │       │   └──  asap_tdt_sortingextractor.py
    │       ├── interfaces
    │       │   ├── __init__.py
    │       │   ├── asap_tdt_eventsinterface.py
    │       │   ├── asap_tdt_filtered_recordinginterface.py
    │       │   ├── asap_tdt_recordinginterface.py
    │       │   └── asap_tdt_sortinginterface.py
    │       ├── metadata
    │       │   ├── ecephys_metadata.yaml
    │       │   ├── embargo_metadata.yaml
    │       │   ├── public_metadata.yaml
    │       │   ├── subjects_metadata.yaml
    │       ├──tutorials
    │       │   └── asap_tdt_demo.ipynb
    │       ├── asap_tdt_convert_all_sessions.py
    │       ├── asap_tdt_convert_session.py
    │       ├── asap_tdt_notes.md
    │       ├── asap_tdt_requirements.txt
    │       ├── asap_tdtnwbconverter.py
    │       └── __init__.py
    └── __init__.py

For the conversion asap_tdt you can find a directory located in src/turner-lab-to-nwb/asap_tdt. Inside the conversion directory you can find the following files:

• asap_tdt_convert_all_sessions.py: this script defines the function to convert all sessions of the conversion. (Note: this script requires the expected folder structure)
• asap_tdt_convert_sesion.py: this script defines the function to convert one full session of the conversion.
• asap_tdt_notes.md: notes and comments concerning this specific conversion.
• asap_tdt_requirements.txt: dependencies specific to this conversion.
• asap_tdtnwbconverter.py: the place where the NWBConverter class is defined.
• extractors: a directory containing the recording and sorting extractors for this specific conversion.
• interfaces: a directory containing the recording and sorting interfaces for this specific conversion.
• metadata: a directory containing the metadata for this specific conversion.
• tutorials: a directory containing tutorials and examples for this specific conversion.

Notes on the conversion

The conversion notes contain information about the expected folder structure and the conversion process.

Running a specific conversion

To run a specific conversion, you might need to install first some conversion specific dependencies that are located in each conversion directory: pip install -r src/turner_lab_to_nwb/asap_tdt/asap_tdt_requirements.txt

You can run a specific conversion with the following command: python src/turner_lab_to_nwb/asap_tdt/asap_tdt_convert_sesion.py

NWB tutorials

The tutorials directory contains Jupyter notebooks that demonstrate how to use the NWB files created by the conversion scripts.

You might need to install jupyter before running the notebooks:

pip install jupyter cd src/turner_lab_to_nwb/asap_tdt/tutorials jupyter lab