https://github.com/danforthcenter/brachypodium-heat-drought-paper
PlantCV python scripts for image analysis; R scripts for data filtering, organizing, and analysis; shell scripts for SNP calling and population structure estimation; input data files for all analyses
https://github.com/danforthcenter/brachypodium-heat-drought-paper
Science Score: 33.0%
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Repository
PlantCV python scripts for image analysis; R scripts for data filtering, organizing, and analysis; shell scripts for SNP calling and population structure estimation; input data files for all analyses
Basic Info
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Metadata Files
README.md
brachypodium-heat-drought-paper
Repository for: Ludwig E, Sumner J, Berry J, Polydore S, Ficor T, Agnew E, Haines K, Greenham K, Fahlgren N, Mockler TC, and Gehan MA. (2023). Natural variation in Brachypodium distachyon responses to combined abiotic stresses. Plant J. http://dx.doi.org/10.1111/tpj.16387
PlantCV python scripts for image analysis; R scripts for data filtering, organizing, and analysis; shell scripts for SNP calling and population structure estimation; input data files for analyses
Abstract: The demand for agricultural production is becoming more challenging as climate change increases global temperature and the frequency of extreme weather events. This study examines the phenotypic variation of 149 accessions of Brachypodium distachyon under drought, heat, and the combination of stresses. Heat alone causes the largest amounts of tissue damage while the combination of stresses causes the largest decrease in biomass compared to other treatments. Notably, Bd21-0, the reference line for B. distachyon, did not have robust growth under stress conditions, especially the heat and combined drought and heat treatments. The climate of origin was significantly associated with B. distachyon responses to the assessed stress conditions. Additionally, a GWAS found loci associated with changes in plant height and the amount of damaged tissue under stress. Some of these SNPs were closely located to genes known to be involved in responses to abiotic stresses and point to potential causative loci in plant stress response. However, SNPs found to be significantly associated with a response to heat or drought individually are not also significantly associated with the combination of stresses. This, with the phenotypic data, suggests that the effects of these abiotic stresses are not simply additive, and the responses to the combined stresses differ from drought and heat alone.
Other data:
Image dataset: https://datacommons.cyverse.org/browse/iplant/home/shared/danforthcenter/LudwigetalBrachypodium Genotype-by-sequencing: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA312869
Owner
- Name: Donald Danforth Plant Science Center
- Login: danforthcenter
- Kind: organization
- Location: St. Louis, MO
- Website: https://danforthcenter.org
- Repositories: 97
- Profile: https://github.com/danforthcenter
Our Mission: Improve the Human Condition Through Plant Science
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| ludwell | 8****l | 90 |
| ludwell | e****g@w****u | 2 |
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