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ix83-modules

Modular inverted microscope for combinatorial imaging technqiues

https://github.com/yiplab/ix83-modules

Science Score: 44.0%

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Repository

Modular inverted microscope for combinatorial imaging technqiues

Basic Info
  • Host: GitHub
  • Owner: YipLab
  • Language: Jupyter Notebook
  • Default Branch: master
  • Homepage:
  • Size: 2.73 MB
Statistics
  • Stars: 0
  • Watchers: 2
  • Forks: 0
  • Open Issues: 0
  • Releases: 3
Created about 6 years ago · Last pushed over 1 year ago
Metadata Files
Readme Citation

README.md

IX83-Modules

Modules enable different microscopy techniques to be applied on the same optical system. Modules are inserted into designated modular spaces the IX83 system. Detailed description of the modules can be found in their respective folders.

System Schematic

The system has 4 free spaced lasers that are aligned using a laser combiner. The beam profile can be manipulated in to modular exciation areas(Mod_Ex). The beam is then expanded and focused to the back focal plane of the objective.

There are two modular emission areas(ModEm), this permits image manipulation before it is acquired by the camera. ModEm1 is the second deck in a dual deck IX83 microscope body. The second is space between the microscope body and the camera.

Components

Lasers

405 Laser | Coherent Cube 405 nm 25 mW
488 Laser | Coherent Sapphire 488 nm 200 mW
561 Laser | Laser Quantum GEM 561 nm 200 mW
647 Laser | Coherent OBIS 637 nm 160 mW

Optics

L1 | Achromatic Doublet f = 30 mm (AC254-030-A-ML)
L2 | Achromatic Doublet f = 250 mm (AC254-250-A-ML)
L3 | Achromatic Doublet f = 300 mm (AC254-300-A-ML)
IX83 | Olympus IX83 Dual Deck

Objectives

5x | Olympus LMPlanFLN 5x/0.13
10x | Olympus UPlanFl 10x/0.30
20x | Olympus LCPlanFl 20x/0.40
40x | Olympus LCPlanFl 40x/0.60
60x | Olympus PlanApo 60x/1.4 Oil
100x | Olympus UApoN 100x/1.49 Oil

Filter Cube

Quad band | Chroma (TRF89901-EMv2-OL3)

Camera

CAM | Photometrics Prime BSI sCMOS

Software

Micromanager 1.4

TODO:

  • software requirements *make folder *shutter code, laser control code, micromanager config
  • license

Owner

  • Name: YipLab
  • Login: YipLab
  • Kind: organization

Citation (CITATION.cff) 

cff-version: 1.2.0
message: "If you use this software, please cite it as below."
authors:
- family-names: "Au"
  given-names: "AARON"
  orcid: "https://orcid.org/my-orcid?orcid=0000-0001-5717-048X"
title: "IX83-Modules"
version: 1.0.0
date-released: 2022-06-22
url: "https://github.com/YipLab/IX83-Modules"

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