12

Try to observe with a microscope what is making an UV/Vis spectroscopy method fail

https://github.com/roberts-farm-of-ideas/12

Science Score: 49.0%

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    Found 5 DOI reference(s) in README
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    Low similarity (8.4%) to scientific vocabulary

Keywords

failure investigate microscopy open-science science-ideas spectroscopy uv-vis
Last synced: 6 months ago · JSON representation

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Try to observe with a microscope what is making an UV/Vis spectroscopy method fail

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  • Host: GitHub
  • Owner: roberts-farm-of-ideas
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failure investigate microscopy open-science science-ideas spectroscopy uv-vis
Created over 4 years ago · Last pushed 6 months ago
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README.md

Robert's Farm of Ideas: 12 -- Try to observe with a microscope what is making an UV/Vis spectroscopy method fail

DOI of this idea License: CC BY 4.0

This idea in short

In the publication 'Spectral Unmixing-Based Reaction Monitoring of Transformations between Nucleosides and Nucleobases' (doi: https://doi.org/10.1002/cbic.202000204) it says:

However, on average in approximately 1–2 % of all measurements, we observed increased absorption across the entire spectrum apparent as a distinct baseline shift (Figure 2C). This results in an inability to obtain accurate fits without manual spectral processing (which we choose to explicitly abstain from), as in these cases the baseline can be shifted >0.10 AU. The straightforward solution in this case is remeasurement of the sample in a different well, which generally resolves the problem.

To check this hypothesis, it would be great to check with a microscope the well, and/or pipette the 'failing' solution into a different well and measure again, and/or ... Any further ideas?

Sounds great, but...

You are interested in this topic? But the idea is gibberish and incomplete, or non-sense? Well, you're probably right!

All of this is work in progress -- I would be very happy if you get in touch so we can improve this!

How to Use

You are free to use this idea in whatever way you like. I put this material under CC-BY license, just to make it legally clear, but: "Gedanken sind frei" (ideas are free) -- I don't expect them to be protectable anyway.

About this Farm of Ideas

This "Farm of Ideas" is supposed to store ideas I would like to develop into scientific progress some day, and to store their progression from initial idea being had, over keeping them alive, to growing them into something real and then "harvesting".

If you are faster in developing them, I am happy with you and for the world! :) Just to avoid me having spend time on something you already realized then, I would be grateful for a short letting-me-know email.

About me

See https://robert-giessmann.de.

Owner

  • Name: Robert's Farm of Ideas
  • Login: roberts-farm-of-ideas
  • Kind: organization
  • Email: farm@robert-giessmann.de
  • Location: Berlin, Germany

This "Farm of Ideas" stores ideas I would like to see developed into scientific progress some day. Please browse and use them!

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