triplecolocalization

Program used to calculate the colocalization between three color channels during immunofluoresence micoscopy. Program will calculate the percentage of overlap of channel 1 to both channels 2 and 3 together. Output colocalization is based on Manders' Colocalization Coefficient

https://github.com/amorante1/triplecolocalization

Science Score: 44.0%

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  • CITATION.cff file
    Found CITATION.cff file
  • codemeta.json file
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  • .zenodo.json file
    Found .zenodo.json file
  • DOI references
  • Academic publication links
  • Academic email domains
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  • JOSS paper metadata
  • Scientific vocabulary similarity
    Low similarity (4.3%) to scientific vocabulary
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Repository

Program used to calculate the colocalization between three color channels during immunofluoresence micoscopy. Program will calculate the percentage of overlap of channel 1 to both channels 2 and 3 together. Output colocalization is based on Manders' Colocalization Coefficient

Basic Info
  • Host: GitHub
  • Owner: amorante1
  • Language: Python
  • Default Branch: master
  • Size: 17.6 KB
Statistics
  • Stars: 1
  • Watchers: 1
  • Forks: 0
  • Open Issues: 1
  • Releases: 1
Created over 3 years ago · Last pushed over 1 year ago
Metadata Files
Readme Citation

README.md

TripleColocalization

Program used to calculate the colocalization between three color channels during immunofluoresence micoscopy. Program will calculate the percentage of overlap of channel 1 to both channels 2 and 3 together. Output colocalization is based on Manders' Colocalization Coefficient. To use this file:

  1. External libraries needed: PIL, xlwt, xlrd, xlutils
  2. Create an empty folder anywhere on computer.
  3. Input your three individual color channel images into the folder.
  4. Start the file and, when prompted, select the folder that contains the three images to analyze.
  5. Adjust the image brightness as necessary with slider bar and press Update button.
  6. Press Analyze button.
  7. Excel sheet should autogenerate in Colocalization folder with mM1, mM2, and mM3 coefficients.

Owner

  • Name: Anthony V. Morante
  • Login: amorante1
  • Kind: user
  • Company: Fordham University

Citation (CITATION.cff)

cff-version: 1.2.0
message: "If you use this software, please cite it as below."
authors:
  - family-names: Morante
    given-names: Anthony V.
    orcid: https://orcid.org/0000-0001-8295-8682
title: TripleColocalization
version: 1.1
doi: 10.5281/zenodo.7368107
date-released: 2022-11-27

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