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panpipes

Multi-modal single cell analysis pipelines

https://github.com/dendroulab/panpipes

Science Score: 67.0%

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    Links to: springer.com
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    Low similarity (15.2%) to scientific vocabulary
Last synced: 6 months ago · JSON representation ·

Repository

Multi-modal single cell analysis pipelines

Basic Info
  • Host: GitHub
  • Owner: DendrouLab
  • License: bsd-3-clause
  • Language: Python
  • Default Branch: main
  • Size: 2.59 MB
Statistics
  • Stars: 59
  • Watchers: 3
  • Forks: 11
  • Open Issues: 39
  • Releases: 13
Created over 3 years ago · Last pushed 8 months ago
Metadata Files
Readme Changelog License Citation

README.md

PyPI version

Panpipes - multimodal single cell pipelines

Overview

Panpipes is a set of computational workflows designed to automate multimodal single-cell and spatial transcriptomic analyses by incorporating widely-used Python-based tools to perform quality control, preprocessing, integration, clustering, and reference mapping at scale. Panpipes allows reliable and customisable analysis and evaluation of individual and integrated modalities, thereby empowering decision-making before downstream investigations.

See our documentation

Panpipes is on Genome Biology!

These workflows make use of cgat-core

Available workflows:

  1. "ingest" : Ingest data and compute quality control metrics
  2. "preprocess" : Filter and normalize per modality
  3. "integration" : Integrate and batch correct using single and multimodal methods
  4. "clustering" : Cluster cells per modality
  5. "refmap" : Map queries against reference datasets
  6. "vis" : Visualize metrics from other pipelines in the context of experiment metadata
  7. "qc_spatial" : Ingest spatial transcriptomics data (Vizgen, Visium) and compute quality control metrics
  8. "preprocess_spatial" : Filtering and normalize spatial transcriptomics data
  9. "deconvolution_spatial" : Deconvolve cell types of spatial transcriptomics slides

Installation and configuration

For detailed installation instructions (including those for Apple Silicon machines), refer to the installation instructions here.

We recommend installing panpipes in a conda environment. We provide a minimal conda config file in pipeline_env.yaml. First, clone this repository and navigate to the root directory of the repository:

bash git clone https://github.com/DendrouLab/panpipes.git cd panpipes

Then, create the conda environment and install the nightly version of panpipes using the following command:

bash conda env create --file=pipeline_env.yaml conda activate pipeline_env pip install -e .

Oxford BMRC Rescomp users find additional advice on the installation here.

Releases

Since panpipes v.1.2.0 the spatial workflows use SpatialData as the data format for spatial assays. Check out the documentation at https://panpipes-pipelines.readthedocs.io

Since panpipes v0.4.0, the ingest workflow expects different headers for the RNA and Protein modalities from the previous releases. Check the example submission file and the documentation for more detailed instructions.

Citation

Curion, F., Rich-Griffin, C., Agarwal, D. et al. Panpipes: a pipeline for multiomic single-cell and spatial transcriptomic data analysis. Genome Biol 25, 181 (2024). doi: https://doi.org/10.1186/s13059-024-03322-7

Contributors

Created by Charlotte Rich-Griffin and Fabiola Curion. Maintained by Fabiola Curion and Sarah Ouologuem. Additional contributors: Sarah Ouologuem, Devika Agarwal, Lilly May, Kevin Rue-Albrecht, Giulia Garcia, Wojciech Lason, Lukas Heumos, Jarne Belien.

Owner

  • Name: DendrouLab
  • Login: DendrouLab
  • Kind: organization

Citation (CITATION.cff) 

cff-version: 1.2.0
message: "If you use this software, please cite it as below:"
authors:
- family-names: "Curion"
  given-names: "Fabiola"
  orcid: "https://orcid.org/0000-0003-2502-8803"
- family-names: "Rich-Griffin"
  given-names: "Charlotte"
  orcid: "https://orcid.org/0000-0001-8212-9542"
- family-names: "Dendrou"
  given-names: "Calliope"
  orcid: "https://orcid.org/0000-0003-1179-4021"
title: "Panpipes: a pipeline for multiomic single-cell and spatial transcriptomic data analysis"
version: 1.0.0
doi: https://doi.org/10.5281/zenodo.11636539
date-released: 2024-06-13
url: "https://github.com/DendrouLab/panpipes"

GitHub Events

Total
  • Create event: 10
  • Release event: 1
  • Issues event: 22
  • Watch event: 7
  • Issue comment event: 19
  • Push event: 108
  • Pull request review comment event: 13
  • Pull request review event: 23
  • Pull request event: 25
  • Fork event: 1
Last Year
  • Create event: 10
  • Release event: 1
  • Issues event: 22
  • Watch event: 7
  • Issue comment event: 19
  • Push event: 108
  • Pull request review comment event: 13
  • Pull request review event: 23
  • Pull request event: 25
  • Fork event: 1

Issues and Pull Requests

Last synced: 6 months ago

All Time
  • Total issues: 7
  • Total pull requests: 7
  • Average time to close issues: 11 months
  • Average time to close pull requests: 25 days
  • Total issue authors: 6
  • Total pull request authors: 5
  • Average comments per issue: 0.71
  • Average comments per pull request: 0.0
  • Merged pull requests: 5
  • Bot issues: 0
  • Bot pull requests: 0
Past Year
  • Issues: 5
  • Pull requests: 7
  • Average time to close issues: N/A
  • Average time to close pull requests: 25 days
  • Issue authors: 4
  • Pull request authors: 5
  • Average comments per issue: 0.0
  • Average comments per pull request: 0.0
  • Merged pull requests: 5
  • Bot issues: 0
  • Bot pull requests: 0
View more stats
Top Authors
Issue Authors
  • bio-la (27)
  • wlason (18)
  • JarneBelien (5)
  • giuliaelgarcia (4)
  • kevinrue (4)
  • Lilly-May (2)
  • Zethson (2)
  • crichgriffin (1)
  • olivermccallion (1)
  • aryaghate (1)
  • canergen (1)
  • SarahOuologuem (1)
  • zvirblyte (1)
  • viktorzou (1)
  • JHYSiu (1)
Pull Request Authors
  • bio-la (32)
  • giuliaelgarcia (21)
  • SarahOuologuem (12)
  • Lilly-May (11)
  • wlason (9)
  • deevdevil88 (7)
  • kevinrue (6)
  • Zethson (4)
  • liuchuan111 (1)
  • vadimnazarov (1)
  • JarneBelien (1)
Top Labels
Issue Labels
enhancement (8) documentation (6) bug (4) help wanted (1)
Pull Request Labels
enhancement (2) documentation (1) bug (1)

Packages

  • Total packages: 1
  • Total downloads:
    • pypi 65 last-month
  • Total dependent packages: 0
  • Total dependent repositories: 0
  • Total versions: 10
  • Total maintainers: 2
pypi.org: panpipes

Panpipes - multimodal single cell pipelines

  • Versions: 10
  • Dependent Packages: 0
  • Dependent Repositories: 0
  • Downloads: 65 Last month
Rankings
Dependent packages count: 7.5%
Average: 38.7%
Dependent repos count: 69.9%
Maintainers (2)
crichgriffin biola.9.c
Last synced: 7 months ago

Dependencies

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