metabolt - MetaBolt 1.0.0 – Turbo Core

First release of MetaBolt⚡, an end-to-end, fully automated Nextflow pipeline that accelerates the recovery of metagenome-assembled genomes (MAGs) from raw metagenomic reads.

This release of pipeline integrates a novel, optimized k-mer strategy to significantly improve assembly efficiency, allowing for rapid, reproducible, and accurate recovery of MAGs from complex microbial communities.

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Key Features

• Optimized de novo metagenome assembly (MEGAHIT) with a reduced k-mer set for faster and more efficient genome reconstruction.

  • Checkout our study of optimization of assembly: https://www.biorxiv.org/content/10.1101/2024.06.08.598064v2.full.pdf

• Fully automated MAG recovery from raw reads to classified bins

• Parallelized, modular workflow built with Nextflow DSL2 for scalability and maintainability

• Preprocessing & QC:

  • Quality assessment using FastQC
  • Adapter trimming and filtering via fastp

• Assembly & Mapping:

  • High-performance assembly with MEGAHIT
  • Read alignment using BWA-MEM and SAMtools

• Genome Binning:

  • MAG reconstruction with MetaBAT2
  • Depth profiling using jgisummarizebamcontigdepths

• Reporting & Summaries:

  • Aggregated workflow summary via MultiQC
  • DAG visualization, trace reports, and software version logs

• Sustainability Reporting:

  • Carbon footprint estimation with nf-co2footprint plugin

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Author

• @muneebdev7

Full Changelog

For detailed changes, improvements, and fixes, see the CHANGELOG.md

- Nextflow
Published by muneebdev7 11 months ago