7

Measuring the invisible: exploiting a two-substrate two-product equilibrium to determine the decomposition rate of one of the products

https://github.com/roberts-farm-of-ideas/7

Science Score: 49.0%

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Keywords

compound decay decomposition decomposition-rate enzyme-reactions equilibrium equilibrium-constants open-science science-ideas substrate unobservability
Last synced: 6 months ago · JSON representation

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Measuring the invisible: exploiting a two-substrate two-product equilibrium to determine the decomposition rate of one of the products

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  • Host: GitHub
  • Owner: roberts-farm-of-ideas
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Topics
compound decay decomposition decomposition-rate enzyme-reactions equilibrium equilibrium-constants open-science science-ideas substrate unobservability
Created almost 5 years ago · Last pushed 6 months ago
Metadata Files
Readme License Zenodo

README.md

Robert's Farm of Ideas: 7 -- Measuring the invisible

DOI License: CC BY 4.0

This idea in short

When a compound, which is unobservable by analytical means, decays/decomposes, one can still determine its decomposition rate by measuring an equilibrium involving this compound. Exemplarily, a two-substrate two-product equilibrium, in this case nucleoside phosphorolysis (nucleoside + phosphate = nucleobase + pentose-1-phosphate) can be used to determine the decomposition rate of one of the products (pentose-1-phosphate).

On the theoretical side, this idea is about generalizing the kinetic vs equilibrium effect, and demonstrating on which scales of equilibrium constants this process works.

On the applied / wet-lab side, a result using this idea is already published (for ribose-1-phosphate) in https://doi.org/10.1002/cphc.202000901

A dataset to write a publication about (for 2'-deoxyribose-1-phosphate) is at: https://doi.org/10.5281/zenodo.3572070

Sounds great, but...

You are interested in this topic? But the idea is gibberish and incomplete, or non-sense? Well, you're probably right!

All of this is work in progress -- I would be very happy if you get in touch so we can improve this!

How to Use

You are free to use this idea in whatever way you like. I put this material under CC-BY license, just to make it legally clear, but: "Gedanken sind frei" (ideas are free) -- I don't expect them to be protectable anyway.

About this Farm of Ideas

This "Farm of Ideas" is supposed to store ideas I would like to develop into scientific progress some day, and to store their progression from initial idea being had, over keeping them alive, to growing them into something real and then "harvesting".

If you are faster in developing them, I am happy with you and for the world! :) Just to avoid me having spend time on something you already realized then, I would be grateful for a short letting-me-know email.

About me

See https://robert-giessmann.de.

Owner

  • Name: Robert's Farm of Ideas
  • Login: roberts-farm-of-ideas
  • Kind: organization
  • Email: farm@robert-giessmann.de
  • Location: Berlin, Germany

This "Farm of Ideas" stores ideas I would like to see developed into scientific progress some day. Please browse and use them!

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  • EndBug/add-and-commit v4 composite
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  • sangonzal/repository-traffic-action v0.1.5 composite