forensic-mirna-analysis - Vibe Coding: AI-assisted Development v1.1.0

• Updated title to include 'Vibe Coding' methodology
• Added comprehensive development transparency documentation
  
• Improved clarity on sample sizes and limitations
• Removed academic overselling language

- Python
Published by gvmfhy 8 months ago

forensic-mirna-analysis - Initial Release: Forensic miRNA Analysis Pipeline

First release of forensic miRNA analysis pipeline.

What This Does

• Processes two public GEO microarray datasets (GSE153135 and GSE49630)
• Applies standard statistical methods to identify miRNAs with differential expression
• Filters results using established forensic criteria
• Provides reproducible analysis code

Analysis Methods

• Standard microarray preprocessing (RMA normalization for CEL files, background correction for GPR files)
• Wilcoxon rank-sum tests for differential expression
• Cohen's d for effect size calculation
• Multiple testing correction (FDR and Bonferroni)
• Filters: p<0.01, fold change >4, Cohen's d >1.5

Results Summary

The analysis identified potential markers for further validation: - Blood: 155 candidates - Semen: 167 candidates
- Saliva: 49 candidates - Vaginal: 22 candidates

Note: These are computational predictions requiring wet-lab validation.

Code Structure

• Python scripts for data analysis
• R script for Affymetrix CEL file processing
• Documentation of all processing steps
• Visualization of results

- Python
Published by gvmfhy 8 months ago