singlem

Novelty-inclusive microbial (and now dsDNA phage) community profiling of shotgun metagenomes

https://github.com/wwood/singlem

Science Score: 49.0%

This score indicates how likely this project is to be science-related based on various indicators:

  • CITATION.cff file
  • codemeta.json file
    Found codemeta.json file
  • .zenodo.json file
    Found .zenodo.json file
  • DOI references
    Found 14 DOI reference(s) in README
  • Academic publication links
  • Committers with academic emails
    5 of 11 committers (45.5%) from academic institutions
  • Institutional organization owner
  • JOSS paper metadata
  • Scientific vocabulary similarity
    Low similarity (6.5%) to scientific vocabulary

Keywords

bioinformatics metagenomics microbiome
Last synced: 6 months ago · JSON representation

Repository

Novelty-inclusive microbial (and now dsDNA phage) community profiling of shotgun metagenomes

Basic Info
Statistics
  • Stars: 161
  • Watchers: 8
  • Forks: 20
  • Open Issues: 41
  • Releases: 0
Topics
bioinformatics metagenomics microbiome
Created almost 11 years ago · Last pushed 6 months ago
Metadata Files
Readme Changelog Citation

README.md

Sandpiper logo Bin Chicken logo Lyrebird logo

Welcome.

At heart, SingleM is a tool for profiling shotgun metagenomes. It was originally designed to determine the relative abundance of bacterial and archaeal taxa in a sample. As of version 0.19.0, it can also be used to profile dsDNA phages (see Lyrebird).

It shows good accuracy in estimating the relative abundances of community members, and has a particular strength in dealing with novel lineages. The method it uses also makes it suitable for some related tasks, such as assessing eukaryotic contamination, finding bias in genome recovery, and lineage-targeted MAG recovery. It can also be used as the basis for choosing metagenomes which, when coassembled, maximise the recovery of novel MAGs (see Bin Chicken).

Microbial SingleM has been applied to ~700,000 public metagenomes. The resulting data are available at the Sandpiper companion website.

Documentation can be found at https://wwood.github.io/singlem/

Citations

Profiling microbial communities with SingleM / Sandpiper

Ben J. Woodcroft, Samuel T. N. Aroney, Rossen Zhao, Mitchell Cunningham, Joshua A. M. Mitchell, Rizky Nurdiansyah, Linda Blackall & Gene W. Tyson. Comprehensive taxonomic identification of microbial species in metagenomic data using SingleM and Sandpiper. Nat Biotechnol (2025). https://doi.org/10.1038/s41587-025-02738-1.

SingleM microbial_fraction

Raphael Eisenhofer, Antton Alberdi, Ben J. Woodcroft, 2024. Large-scale estimation of bacterial and archaeal DNA prevalence in metagenomes reveals biome-specific patterns. bioRxiv, pp.2024-05; https://doi.org/10.1101/2024.05.16.594470.

SingleM-powered coassembly with Bin Chicken

Samuel T. N. Aroney, Rhys J. Newell, Gene W. Tyson and Ben J. Woodcroft, 2024. Bin Chicken: targeted metagenomic coassembly for the efficient recovery of novel genomes. bioRxiv, pp.2024-11. https://doi.org/10.1101/2024.11.24.625082.

Lyrebird

Rossen Zhao, Gene W. Tyson, Ben J. Woodcroft. Lyrebird: a tool for profiling dsDNA phage communities in metagenomic data. (in preparation).

Owner

  • Name: Ben J Woodcroft
  • Login: wwood
  • Kind: user
  • Location: Brisbane, University of Queensland
  • Company: Queensland University of Technology

Informatics team leader at the Centre for Microbiome Research (CMR)

GitHub Events

Total
  • Create event: 16
  • Release event: 1
  • Issues event: 30
  • Watch event: 20
  • Delete event: 6
  • Member event: 1
  • Issue comment event: 107
  • Push event: 84
  • Pull request review event: 32
  • Pull request review comment event: 47
  • Pull request event: 46
  • Fork event: 3
Last Year
  • Create event: 16
  • Release event: 1
  • Issues event: 30
  • Watch event: 20
  • Delete event: 6
  • Member event: 1
  • Issue comment event: 107
  • Push event: 84
  • Pull request review event: 32
  • Pull request review comment event: 47
  • Pull request event: 46
  • Fork event: 3

Committers

Last synced: 10 months ago

All Time
  • Total Commits: 1,472
  • Total Committers: 11
  • Avg Commits per committer: 133.818
  • Development Distribution Score (DDS): 0.196
Past Year
  • Commits: 132
  • Committers: 4
  • Avg Commits per committer: 33.0
  • Development Distribution Score (DDS): 0.311
Top Committers
Name Email Commits
Ben Woodcroft d****n@g****m 1,183
AroneyS s****y@q****u 142
Ben J. Woodcroft d****m 107
rzhao-2 r****o@g****m 31
EisenRa r****r@g****m 3
thepatientwait 9****t 1
Aaron Mussig a****g@g****m 1
rzhao-2 s****5@m****u 1
rzhao-2 s****5@d****u 1
Tim Lamberton t****n@u****u 1
Rossen Zhao z****3@c****u 1
Committer Domains (Top 20 + Academic)

Issues and Pull Requests

Last synced: 6 months ago

All Time
  • Total issues: 122
  • Total pull requests: 112
  • Average time to close issues: 3 months
  • Average time to close pull requests: 17 days
  • Total issue authors: 82
  • Total pull request authors: 6
  • Average comments per issue: 2.75
  • Average comments per pull request: 1.15
  • Merged pull requests: 85
  • Bot issues: 0
  • Bot pull requests: 0
Past Year
  • Issues: 25
  • Pull requests: 44
  • Average time to close issues: 3 days
  • Average time to close pull requests: 8 days
  • Issue authors: 22
  • Pull request authors: 4
  • Average comments per issue: 1.32
  • Average comments per pull request: 1.25
  • Merged pull requests: 29
  • Bot issues: 0
  • Bot pull requests: 0
Top Authors
Issue Authors
  • adityabandla (9)
  • LiZhihua1982 (3)
  • slambrechts (3)
  • rzhao-2 (3)
  • rachaellappan (3)
  • pm-leung (3)
  • lauramason326 (3)
  • michoug (3)
  • wwood (3)
  • xvazquezc (2)
  • ellyyuyang (2)
  • Valentin-Bio (2)
  • julianzaugg (2)
  • kjvilardi (2)
  • Ge0rges (2)
Pull Request Authors
  • AroneyS (47)
  • rzhao-2 (44)
  • wwood (12)
  • thepatientwait (6)
  • EisenRa (2)
  • aaronmussig (1)
Top Labels
Issue Labels
Pull Request Labels
codex (5)

Packages

  • Total packages: 1
  • Total downloads:
    • pypi 113 last-month
  • Total dependent packages: 0
  • Total dependent repositories: 1
  • Total versions: 22
  • Total maintainers: 2
pypi.org: singlem

Novelty-inclusive microbial community profiling of shotgun metagenomes

  • Versions: 22
  • Dependent Packages: 0
  • Dependent Repositories: 1
  • Downloads: 113 Last month
Rankings
Stargazers count: 8.0%
Forks count: 9.6%
Dependent packages count: 10.0%
Average: 13.3%
Downloads: 17.0%
Dependent repos count: 21.7%
Maintainers (2)
Last synced: 6 months ago