dsuz_popgen_gea

Population genomic and GEA analyses on Drosophila suzukii

https://github.com/sfeng666/dsuz_popgen_gea

Science Score: 44.0%

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Repository

Population genomic and GEA analyses on Drosophila suzukii

Basic Info
  • Host: GitHub
  • Owner: Sfeng666
  • License: mit
  • Language: Python
  • Default Branch: main
  • Size: 7.88 MB
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  • Watchers: 1
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Created over 2 years ago · Last pushed 11 months ago
Metadata Files
Readme License Citation

README.md

DsuzpopgenGEA: population genomics and GEA analyses on Drosophila suzukii


Code for major analyses performed in population genomic analyses and genome-environment association (GEA) analyses on Drosophila suzukii.

Related publication:

Feng S, DeGrey SP, Guédot C, Schoville SD, Pool JE. 2024. Genomic Diversity Illuminates the Environmental Adaptation of Drosophila suzukii. Genome Biology and Evolution 16:evae195.

Analyses list

  1. Identify autosomal and X-linked contigs
  2. Annotate genomic features
  3. Calculate sequence divergence between two species
  4. Estimate genome-wide and synonymous nucleotide diversity, and illustrate window diversity across chromosome arms
  5. Estimate genome-wide and synonymous FST
  6. Estimate genome-wide and synonymous DXY
  7. Perform PCA on allele frequencies
  8. Build population tree and infer admixture from allele frequency
  9. Perform genome-environment association (GEA) analysis
  10. Identify environment-associated genes
  11. Perform GO enrichment of the top environment-associated genes

Environment setup

To set up the environment for above analyses, you could use conda: conda env create -n environemnt_name --file environemnt_name.yml Environment needed for each analysis is indicated in documentation of each analysis. Environmental package lists (environemnt_name.yml) for cross-platform install could be found under this directory.

If you have not installed conda, run the following command: ```

download miniconda

curl -sL \ "https://repo.anaconda.com/miniconda/Miniconda3-latest-Linux-x86_64.sh" > \ "Miniconda3.sh"

install miniconda

bash Miniconda3.sh ```

Owner

  • Name: Siyuan Feng
  • Login: Sfeng666
  • Kind: user
  • Location: Madison, WI
  • Company: University of Wisconsin-Madison

Evolutionary biologist working on population genomics & comparative transcriptomics

Citation (CITATION)

To reference poolWGS2SNP in publications, please cite:

@article {Feng2023.07.03.547576,
        author = {Siyuan Feng and Samuel P. DeGrey and Christelle Gu{\'e}dot and Sean D. Schoville and John E. Pool},
        title = {Genomic Diversity Illuminates the Environmental Adaptation of Drosophila suzukii},
        elocation-id = {2023.07.03.547576},
        year = {2023},
        doi = {10.1101/2023.07.03.547576},
        publisher = {Cold Spring Harbor Laboratory},
        URL = {https://www.biorxiv.org/content/early/2023/07/03/2023.07.03.547576},
        eprint = {https://www.biorxiv.org/content/early/2023/07/03/2023.07.03.547576.full.pdf},
        journal = {bioRxiv}
}

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