Bio-Ernst-HK-Stelzer

Curriculum Vitae; CV; Bio; Lebenslauf; Scientific Profile; Literature; Products; Patents; References

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Invention of Light Sheet Fluorescence Microscopy (LSFM)

Light sheet fluorescence microscopy (LSFM), also known as single/selective plane illumination microscopy (SPIM), represents a significant advancement in the field of fluorescence microscopy. The technique is distinguished by its innovative use of a sheet of light to selectively illuminate a plane of the specimen, thereby minimizing photodamage and photobleaching while enabling high-resolution, long-term imaging of live specimens.

Key Contributions

The conceptual foundation and development of LSFM are credited to Ernst H.K. Stelzer and his team at the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany. Their pioneering work has been instrumental in demonstrating the technique's potential and optimizing it for biological research applications.

Advancements and Impact

• Minimized Photodamage: LSFM significantly reduces the exposure of specimens to damaging light, allowing for longer observation periods without compromising the health of the specimen.
• Enhanced Imaging: The technique provides high-resolution images of live specimens, making it an invaluable tool for studying dynamic biological processes, especially in developmental biology.
• Broad Applications: Since its introduction, LSFM has revolutionized various research fields by offering a powerful tool for detailed and dynamic imaging of complex biological systems.

Outlook

The invention and refinement of Light Sheet Fluorescence Microscopy by Ernst H.K. Stelzer and colleagues mark a pivotal moment in microscopy and biological imaging. Through their efforts, LSFM has emerged as a cornerstone technique that continues to expand our understanding of life at the microscopic level.

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Curriculum Vitae

Prof. Dr. Ernst Hans Karl Stelzer

| Personal Details | | | :-------- | -------: | | Family Name: | Stelzer | | Given Name: | Ernst Hans Karl | | Date of Birth: | January 5, 1959 | | Place of Birth: | Frankfurt am Main, Germany | | Nationality: | German | | Family Status: | Married, two children and two grandchildren | | Private Address: | Meckesheim close to Heidelberg, Germany | | Phone @ Goethe-Universität: | +49 (69) 798 42547 | Email: ernst.stelzer@physikalischebiologie.de | | E-Mail @ Goethe-Universität: | ernst.stelzer@physikalischebiologie.de |

Current Positions

• W3 Professor, Vice Dean (Research), FB15
• Affiliation, Goethe-Universität Frankfurt am Main, Campus Riedberg, Max-von-Laue-Straße 15, D-60438 Frankfurt am Main, Germany
• Director, Frankfurt Center for Advanced Light Microscopy
• Co-Speaker, RobustNature
• Board, Center for Critical Computational Studies

Education

• Ph.D., European Molecular Biology Laboratory (EMBL-Heidelberg), 1987 in the Group of Dr. Roelof Wijnaendts-van-Resandt. Supervised by Prof. Dr. Bille (Universität Heidelberg, Angewandte Physik) and Dr. rer. nat. habil. Männer (Physikalisches Institut).
• Diploma in Physics, Johann-Wolfgang-Goethe-Universität, Frankfurt am Main, Germany, 1983 in the Group of Dr. Ernst Grell. Thesis at Max-Planck-Institut für Biophysik, Frankfurt am Main.

Professional Career Highlights

• Professor at Goethe-Universität Frankfurt am Main, since 2011.
• Offer W3 Professorship by Goethe-Universität Frankfurt am Main, 2009.
• Scientific Group Leader at European Molecular Biology Laboratory (EMBL-Heidelberg), 1989-2011.
• Assistant, Max-Planck-Institut für Biophysik, Frankfurt am Main, Germany, 1979-1983.

Scientific Profile

• Fields of Expertise: Physical Biology, Cell Biology, Developmental Biology, Optics, Physics, and Statistics.
• Main Research Interests: Three-dimensional Light Microscopy, Advanced Data & Image Analysis, Dynamic Imaging of Multi-cellular Biological Systems.

Professional Memberships

• Deutsche Physikalische Gesellschaft (DPG)
• Optical Society of America (OSA, Washington, USA)
• Society of Photo-Optical Instrumentation Engineers (SPIE, Bellingham, USA)

Honours and Prizes

• Highly Cited Researcher, 2018 (Clarivate Analytics)
• Lennart Philipson Award, European Molecular Biology Laboratory (EMBL), 2016
• Honorary Fellow, Royal Microscopical Society, 2014
• Protagonist Method of the Year 2014, Nature Methods, January 2015
• EMBO Fellow, European Molecular Biology Organisation, 2009

Selected Community Services

• Member, EMBL Alumni Board, 2020-2023
• Dean of Education, Goethe-Universität FB15, 2017-2019
• Dean of Research, Goethe-Universität FB15, since 2019

Significant Publications

Some important publications:

1. Stelzer EHK, Strobl F, Chang BJ, Preusser F, Preibisch S, McDole K, Fiolka R (2021) Light sheet fluorescence microscopy. Nature Reviews Methods Primers 1, 73. doi.org/10.1038/s43586-021-00069-4.
2. Strobl F, Stelzer EHK (2021) A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds. Sci Rep 11(15325). doi.org/10.1038/s41598-021-94288-0.
3. Gebel J, Tuppi M, Chaikuad A, Hötte K, Schröder M, Schulz L, Löhr F, Gutfreund N, Finke F, Henrich E, Mezhyrova J, Lehnert R, Pampaloni F, Hummer G, Stelzer EHK, Knapp S, Dötsch V (2020) p63 uses a switch-like mechanism to set the threshold for induction of apoptosis. Nature Chemical Biology 16(10):1078-1086. doi: 10.1038/s41589-020-0600-3. PMID: 32719556.
4. von Wangenheim D, Banda J, Schmitz A, Boland J, Bishopp A, Maizel A, Stelzer EHK, Bennett M (2020) Early developmental plasticity of lateral roots in response to asymmetric water availability. Nature Plants 6(2):73-77. doi: 10.1038/s41477-019-0580-z. PMID: 32015516.
5. Pampaloni F, Reynaud EG, Stelzer EHK (2007) The third dimension bridges the gap between cell culture and live tissue, Nature Reviews Molecular Cell Biology 8(10):839-845.
6. Chang BJ, Perez Meza VD, Stelzer EHK (2017) csiLSFM combines light-sheet fluorescence microscopy and coherent structured illumination for a lateral resolution below 100 nm, PNAS 114(19):4869-4874.
7. Stelzer EHK (2015) Light-sheet fluorescence microscopy for quantitative biology, Nature Methods, 12(1):23–27.
8. Keller PJ, Schmidt AD, Santella A, Khairy K, Bao Z, Wittbrodt J, Stelzer EHK (2010) Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy, Nature Methods 7(8):637-642.
9. Keller PJ, Schmidt AD, Wittbrodt J, Stelzer EHK (2008) Reconstruction of zebrafish early embryonic development by Scanned Light Sheet Microscopy, Science, 322(5904):1065-1069.
10. Huisken J, Swoger J, Del Bene F, Wittbrodt J, Stelzer EHK (2004) Optical sectioning deep inside live embryos by selective plane illumination microscopy, Science 305:1007-1009.
11. Stelzer EHK, Lindek S (1994) Fundamental reduction of the observation volume in far-field light microscopy by detection orthogonal to the illumination axis: confocal theta microscopy. Optics Communications 111:536-547.
12. Hell S, Stelzer EHK (1992) Properties of a 4Pi confocal fluorescence microscope. Journal of the Optical Society of America A, 9(12), 1259–2166.

Metrics and Impact

• Web of Science ResearcherID: A-7648-2011 | Sum citations: >18,000 (without self-citations) | h-index: 64 (>270 publications).
• Google Scholar: EV5RvqkAAAAJ | Sum citations: >33,800 | h-index: 82. Google Scholar.
• ORCID: 0000-0003-1545-0736.

Third-party Funding (Last Ten Years)

Secured a total of €12 million in funding from diverse sources, including the CEF-MC I&II, the European Union (3×EU), the German Research Foundation (4×DFG), the Federal Ministry of Education and Research (3×BMBF), and the State Offensive for the Development of Scientific and Economic Excellence (2×LOEWE).

Biography

Ernst H.K. Stelzer is a luminary in the domain of Physical Biology and Advanced Light Microscopy, holding a professorship at Goethe-Universität, Frankfurt am Main, Hessen, Germany since March 2011. His appointment marked the establishment of a new professorship integrated into two Clusters of Excellence for Macromolecular Complexes (CEF-MC I & II), sponsored by the German Research Council (DFG). Prof. Stelzer's research endeavors focus on the application and advancement of light microscopy technologies in life sciences, specifically in the areas of three-dimensional cell biology and the embryogenesis of various insects, including Tribolium castaneum. His innovations have empowered researchers to observe and manipulate biological specimens with unprecedented precision and resolution.

From 1987 to 2011, Prof. Stelzer distinguished himself as a Scientific Group Leader at the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany, contributing significantly to the Cell Biology and Biophysics Unit. His extensive work spans several disciplines, including physics, optics, biophysics, cell biology, molecular biology, and developmental biology. Prof. Stelzer is particularly celebrated for his foundational contributions to conventional and confocal fluorescence microscopy, 4Pi- and theta-microscopy, optical tweezers and levitation, laser ablation, and the pioneering development of light sheet-based fluorescence microscopy (LSFM).

His scholarly publications have profoundly impacted the fields of optics, biophysics, cell biology, developmental biology, and plant biology. Prof. Stelzer's commitment to developing three-dimensional imaging techniques has facilitated the observation of complex biological systems in near-natural states over time. A mentor to many, his former students and postdocs have gone on to forge successful careers in both academia and industry. To date, he has published over 270 papers and secured numerous patents, safeguarding the intellectual property rights of several commercially available optical instruments.

In recognition of his groundbreaking work, light sheet-based fluorescence microscopy was acclaimed as the "Method of the Year 2014" by Nature Methods. Prof. Stelzer's career is decorated with numerous awards and honors, reflecting his substantial contributions to the scientific community.

Websites for Further Information

• Physikalische Biologie
• RobustNature

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This restructured and enhanced curriculum vitae presents Prof. Dr. Ernst Hans Karl Stelzer not only as a distinguished academic and researcher but also highlights his global impact on the fields of physical biology and microscopy. His contributions extend beyond academia, influencing the development of technologies that enhance our understanding of biological systems at the microscale.

Certainly! Below is the requested list, formatted in Markdown:

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Selection Patents Related to CFM, PFM and LSFM

1. Konfokales Mikroskop (Confocal Microscope)

   • Inventors: Stelzer EHK, Lindek S, Pick R
     
   • Patent Office: Deutsches Patent- und Markenamt
     
   • Patent Number: DE 43 26 473
     
   • Filing Date: 6 August 1993
     
   • Grant Date: 6 December 1996
     
   • Publication Date of Grant: 15 May 1997

2. Konfokales Mikroskop mit Doppelobjektiv-System (Confocal Microscope with Dual Objective System)

   • Inventors: Stelzer EHK, Lindek S
     
   • Patent Office: Deutsches Patent- und Markenamt
     
   • Patent Number: DE 196 29 725
     
   • Filing Date: 23 July 1996
     
   • Grant Date: 19 December 1997
     
   • Disclosure Date: 5 February 1998

3. Strahlumlenkeinheit zur mehrachsigen Untersuchung in einem Mikroskop (Beam Diverter Unit for Multiaxial Investigation in a Microscope)

   • Inventors: Stelzer EHK, Lindek S
     
   • Patent Office: European Patent Office
     
   • Patent Number: EP 0 859 968
     
   • Filing Date: 22 July 1997
     
   • Publication and Announcement of Grant: 25 February 2004 (Patentblatt 2004/09)

4. Konfokales Theta-Mikroskop (Confocal Theta-Microscope)

   • Inventors: Stelzer EHK, Lindek S, Stefany T, Swoger J
     
   • Patent Office: Europäisches Patentamt
     
   • Patent Number: EP 1 019 769 B1
     
   • Filing Date: 27 July 1999
     
   • Publication and Announcement of Grant: 29 September 2004 (Patentblatt 2004/40)

5. Verfahren und Anordnung zur konfokalen Mikroskopie (Method and Arrangement for Confocal Microscopy)

   • Inventors: Tille S, Simon U, Möhler G, Wilhelm S, Meisel U, Stelzer EHK
     
   • Patent Office: European Patent Office
     
   • Patent Number: EP 0 977 069 A2
     
   • Filing Date: 1 July 1999

6. Verfahren zur dreidimensionalen Abtastung (Method for Three-Dimensional Scanning)

   • Inventors: Florin EL, Hörber JKH, Stelzer EHK
     
   • Patent Office: Deutsches Patent- und Markenamt
     
   • Patent Number: DE 199 39 574
     
   • Filing Date: 27 August 1999

7. Verfahren und Anordnung zur konfokalen Mikroskopie (Method and Arrangement for Confocal Microscopy)

   • Inventors: Stelzer EHK, Stefany T, Lindek S, Swoger J
     
   • Patent Office: Deutsches Patent- und Markenamt
     
   • Patent Number: 198 61 363.6 (Division of parent patent DE 198 29 981.8)
     
   • Filing Date: 25 February 2005

8. Microscope with a Viewing Direction Perpendicular to the Illumination Direction

   • Inventors: Stelzer EHK, Huisken J, Enders S, Swoger J, Lindek S
     
   • Patent Office: Europäisches Patentamt
     
   • International Application Number: PCT/EP 03/05991
     
   • Filing Date: 6 June 2003
     
   • International Publication Number: WP 2004/053558 A1
     
   • International Publication Date: 24 June 2004

9. Microscope with a Viewing Direction Perpendicular to the Illumination Direction

   • Inventors: Stelzer EHK, Enders S, Huisken J, Lindek S, Swoger J
     
   • Patent Office: United States Patent Office
     
   • Patent Number: US 7,554,725 B2
     
   • Date of Patent: 30 June 2009
   • Application No.: US 10/538,081
     
   • PCT Filed: 6 June 2003
     
   • PCT No.: PCT/EP03/05991

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