claidenttutorial
Science Score: 26.0%
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Low similarity (8.0%) to scientific vocabulary
Repository
Basic Info
- Host: GitHub
- Owner: astanabe
- License: gpl-2.0
- Language: Shell
- Default Branch: main
- Size: 716 MB
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- Stars: 0
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Metadata Files
README.md
ClaidentTutorial
Tutorial scripts and data for Claident.
Lecture video is available
- (Introduction to metabarcoding: The reality of dream technology) Speech in Japanese with English slides
- ClaidentRDNA (Lecture course on environmental DNA metabarcoding using Claident and R: From nucleotide sequence data processing to ecological analyses) Speech in Japanese with English slides and auto-translated English subtitles
- ClaidentRDNA (Lecture course on "quantitative" environmental DNA metabarcoding using Claident and R: From nucleotide sequence data processing to ecological analyses) Speech in Japanese with English slides
Prerequisites to run Claident
Claident, associated databases and programs need to be installed. Use ClaidentInstaller to install them. In addition, this package need to be downloaded and extracted to working directory. For wordcloud image generation ("clplotwordcloud" command), Google Chrome or Chromium is also required.
Prerequisites to run analyses in R
R and several packages need to be installed. Install R, and then, execute the following command in R
library(parallel)
install.packages(c("vegan", "colorspace", "RColorBrewer", "tidyverse", "ggsci", "khroma", "picante", "bipartite", "geosphere", "foreach", "doParallel", "mpmcorrelogram", "pvclust"), repos="http://cloud.r-project.org/", clean=T, upgrade="never", Ncpus=detectCores())
You might need to install several programs and/or libraries such as libssl or libgdal before executing the above package installation in R. If you are trying to run this tutorial on Ubuntu or Debian, the following command may helps you.
sudo apt install libssl-dev libgdal-dev
Prerequisites to learn about Claident and R
Just download this package and extract to working directory.
Learning how to run Claident
See the following shell scripts, inputs and outputs.
- runClaidentsingleendwoSTD.sh
- runClaidentoverlappedpairedendwoSTD.sh
- runClaidentnonoverlappedpairedendwoSTD.sh
- runClaidentoverlappedpairedendwSTD.sh
Learning how to analyze Claident outputs in R
See the following R codes, inputs and outputs.
Owner
- Name: Akifumi S. Tanabe
- Login: astanabe
- Kind: user
- Location: Sendai, Miyagi, Japan
- Company: Graduate School of Life Sciences, Tohoku University
- Website: https://www.fifthdimension.jp/
- Repositories: 1
- Profile: https://github.com/astanabe
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