temperature_gradient_microscopy_stage
Microscopy stage with two temperature controlled metal block and a translational frame to move a sample in the temperature gradient.
https://github.com/dogerber/temperature_gradient_microscopy_stage
Science Score: 49.0%
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Repository
Microscopy stage with two temperature controlled metal block and a translational frame to move a sample in the temperature gradient.
Basic Info
- Host: GitHub
- Owner: dogerber
- License: mit
- Language: C++
- Default Branch: main
- Size: 18 MB
Statistics
- Stars: 4
- Watchers: 1
- Forks: 2
- Open Issues: 0
- Releases: 3
Metadata Files
README.md
Temperature Gradient Microscopy Stage
Microscopy stage with two temperature controlled metal block and a translational frame to move a sample in the temperature gradient by D. Gerber from the (now retired) Soft and Living Materials Group at ETH Zrich.
Detailed instructions to build the setup can be found in the wiki.
This has been used for the following publications: * Stress accumulation by confined ice in a temperature gradient (PNAS) * Polycrystalinity enhances stress build-up around ice (PRL) * Dehydration drives damage in the freezing of brittle hydrogels
Setup overview
The setup consists of a PID control loop, where an Arduino microcontroller regulates the voltage for two peltier devices. Thermistors are placed in two copper blocks of variable spacing above the peltier devices and the opposite side of the peltier devices is held at room temperature by water cooling. The sample is placed on the copper block and can be moved relative to the temperature gradient by a linear actuator.
Stage head
Controller box
Schematic
Features
- 0.05 K temperature accuracy, when calibrated
- 0.01 K temperature stability
- Temperature range -25 to 60 C (colder possible, if using coolant below room temperature)
- Variable spacing between metal blocks of 0-1 cm
- Translation of sample in the temperature gradient with 1.9 cm travel range and 300 nm stepsize
- Optional datalogging to microSD-card
- Optimized for microscope stages with 160x110 mm opening "Universal insert" I-3091, but can be adapted
Acknowledgement
Lawrence Wilen, Christian Furrer and Robert Style helped with the design of this stage and controller. The menu system used is GEM by Spirik.
Inspiration for this stage was also taken from Prof. Sylvain Devilles group.
Owner
- Login: dogerber
- Kind: user
- Repositories: 1
- Profile: https://github.com/dogerber