MAST

Tools and methods for analysis of single cell assay data in R

https://github.com/rglab/mast

Science Score: 23.0%

This score indicates how likely this project is to be science-related based on various indicators:

  • CITATION.cff file
  • codemeta.json file
  • .zenodo.json file
  • DOI references
    Found 1 DOI reference(s) in README
  • Academic publication links
  • Committers with academic emails
    2 of 18 committers (11.1%) from academic institutions
  • Institutional organization owner
  • JOSS paper metadata
  • Scientific vocabulary similarity
    Low similarity (15.6%) to scientific vocabulary

Keywords from Contributors

bioconductor-package genomics gene bioinformatics copy-number cell-free-dna human-cell-atlas recount junction illumina
Last synced: 10 months ago · JSON representation

Repository

Tools and methods for analysis of single cell assay data in R

Basic Info
  • Host: GitHub
  • Owner: RGLab
  • Language: R
  • Default Branch: devel
  • Size: 97.1 MB
Statistics
  • Stars: 249
  • Watchers: 9
  • Forks: 57
  • Open Issues: 38
  • Releases: 3
Created almost 14 years ago · Last pushed almost 3 years ago
Metadata Files
Readme

README.md

MAST: Model-based Analysis of Single-cell Transcriptomics

MAST fits two-part, generalized linear models that are specially adapted for bimodal and/or zero-inflated single cell gene expression data.

Examples and vignettes

MAST supports:

  • Easy importing, subsetting and manipulation of expression matrices
  • Filtering of low-quality cells
  • Adaptive thresholding of background noise
  • Tests for univariate differential expression, with adjustment for covariates
  • Gene set enrichment analysis, corrected for covariates and gene-gene correlations
  • Exploration of gene-gene correlations and co-expression

Vignettes are available in the package via vignette('MAITAnalysis'), vignette('MAST-Intro') or vignette('MAST-interoperability').

New Features and announcements

  • MAST has been ported to use SingleCellExperiment under the hood, and is in Bioconductor.
  • We now make an effort to track assay contents (counts vs log counts). This should facilitate interaction with Scater and SCRAN.

Getting Help

For general questions, please submit a question to the bioconductor support site so that others can benefit from the discussion.

For bug reports (something seems broken): open a bug report here.

Installation Instructions

This version available here on github may only properly function if you are running Bioconductor Devel, which is not something you will want to run for existing analyses! Instead follow instructions below.

You may install or update MAST with:

install.packages("BiocManager") # Needed to install all Bioconductor packages
BiocManager::install("MAST")

Citation

If you find MAST useful in your work, please consider citing the paper: MAST: a flexible statistical framework for assessing transcriptional changes and characterizing heterogeneity in single-cell RNA sequencing data G Finak, A McDavid, M Yajima, J Deng, V Gersuk, AK Shalek, CK Slichter et al Genome biology 16 (1), 278

The version that was used in the Genome Biology paper is accesible under the branch MASTClassic.

Converting old MASTClassic SingleCellAssay objects

If you have data analyzed using MASTClassic, you can convert objects from MASTClassic format to the new format based on SingleCellExperiment using convertMastClassicToSingleCellAssay().

Owner

  • Name: RGLab
  • Login: RGLab
  • Kind: organization
  • Location: Seattle

Raphael Gottardo's Research Lab

GitHub Events

Total
  • Issues event: 4
  • Watch event: 17
  • Issue comment event: 4
Last Year
  • Issues event: 4
  • Watch event: 17
  • Issue comment event: 4

Committers

Last synced: over 2 years ago

All Time
  • Total Commits: 182
  • Total Committers: 18
  • Avg Commits per committer: 10.111
  • Development Distribution Score (DDS): 0.747
Past Year
  • Commits: 12
  • Committers: 5
  • Avg Commits per committer: 2.4
  • Development Distribution Score (DDS): 0.667
Top Committers
Name Email Commits
Andrew McDavid a****d@g****m 46
Greg Finak g****k@g****m 35
Andrew McDavid a****d@f****g 27
Andrew McDavid A****d@u****u 27
Nitesh Turaga n****a@g****m 18
Greg Finak g****k@f****g 4
Andrew McDavid a****w@o****m 4
Herve Pages h****s@f****g 4
Hervé Pagès h****s@f****g 3
vobencha v****a@g****m 2
Jacob Wagner j****r@f****g 2
vobencha v****n@r****g 2
J Wokaty j****y@s****u 2
J Wokaty j****y 2
Sean Hughes s****n@g****m 1
Martin Morgan m****n@f****g 1
Daniel Burkhardt b****b@g****m 1
Derrik Gratz 4****z 1
Committer Domains (Top 20 + Academic)

Packages

  • Total packages: 3
  • Total downloads:
    • bioconductor 211,128 total
  • Total dependent packages: 6
    (may contain duplicates)
  • Total dependent repositories: 0
    (may contain duplicates)
  • Total versions: 7
  • Total maintainers: 1
bioconductor.org: MAST

Model-based Analysis of Single Cell Transcriptomics

  • Versions: 5
  • Dependent Packages: 6
  • Dependent Repositories: 0
  • Downloads: 211,128 Total
Rankings
Dependent repos count: 0.0%
Dependent packages count: 0.0%
Forks count: 0.7%
Stargazers count: 0.9%
Average: 2.1%
Downloads: 9.0%
Last synced: 11 months ago
proxy.golang.org: github.com/RGLab/MAST
  • Versions: 1
  • Dependent Packages: 0
  • Dependent Repositories: 0
Rankings
Dependent packages count: 5.5%
Average: 5.7%
Dependent repos count: 5.8%
Last synced: 11 months ago
proxy.golang.org: github.com/rglab/mast
  • Versions: 1
  • Dependent Packages: 0
  • Dependent Repositories: 0
Rankings
Dependent packages count: 5.5%
Average: 5.7%
Dependent repos count: 5.8%
Last synced: 11 months ago

Dependencies

DESCRIPTION cran
  • R >= 3.5 depends
  • SingleCellExperiment >= 1.2.0 depends
  • Biobase * imports
  • BiocGenerics * imports
  • S4Vectors * imports
  • SummarizedExperiment >= 1.5.3 imports
  • abind * imports
  • data.table * imports
  • ggplot2 * imports
  • graphics * imports
  • methods * imports
  • parallel * imports
  • plyr * imports
  • progress * imports
  • reshape2 * imports
  • stats * imports
  • stats4 * imports
  • stringr * imports
  • utils * imports
  • BiocStyle * suggests
  • DelayedArray * suggests
  • GGally * suggests
  • GSEABase * suggests
  • HDF5Array * suggests
  • Matrix * suggests
  • NMF * suggests
  • RColorBrewer * suggests
  • TxDb.Hsapiens.UCSC.hg19.knownGene * suggests
  • blme * suggests
  • car * suggests
  • dplyr * suggests
  • gdata * suggests
  • knitr * suggests
  • lattice * suggests
  • limma * suggests
  • lme4 >= 1.0 suggests
  • numDeriv * suggests
  • rmarkdown * suggests
  • roxygen2 > 6.0.0 suggests
  • rsvd * suggests
  • scater * suggests
  • testthat * suggests
  • zinbwave * suggests