TitanCNA
Analysis of subclonal copy number alterations (CNA) and loss of heterozygosity (LOH) in cancer
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Analysis of subclonal copy number alterations (CNA) and loss of heterozygosity (LOH) in cancer
Basic Info
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- Stars: 97
- Watchers: 3
- Forks: 37
- Open Issues: 27
- Releases: 0
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Metadata Files
README.md
TitanCNA
TitanCNA a R/Bioconductor package for analyzing subclonal copy number alterations (CNA) and loss of heterozygosity (LOH) in whole genome and exome sequencing of tumours.
Ha, G., et al. (2014). TITAN: Inference of copy number architectures in clonal cell populations from tumour whole genome sequence data. Genome Research, 24: 1881-1893. (PMID: 25060187)
Contact
Gavin Ha
Fred Hutchinson Cancer Research Center
contact: gavinha@gmail.com or gha@fredhutch.org
Date: May 30, 2019
Website: GavinHaLab.org
Table of Contents
Links
Snakemake Workflow: https://github.com/gavinha/TitanCNA/tree/master/scripts/snakemake
10X Snakemake Workflow: https://github.com/gavinha/TitanCNA10Xsnakemake
Google Groups: https://groups.google.com/forum/#!forum/titancna
Publication in Genome Research: http://genome.cshlp.org/content/24/11/1881
News
(See NEWS for previous version notes)
May 30, 2019 - TitanCNA version 1.23.1
Addressed the issue of RangedData being deprecated by converting code to use GRanges from the GenomicRanges package.
New or modified functions:
- wigToGRanges: to load WIG files and store in GRanges object.
- correctIntegerCN(): performs allelic copy number (major/minor CN) adjustment.
August 9, 2018
Improved parameter inference by handling errors and allowing EM to continue until convergence. This fixes runs that previously would fail because samples had very low tumor content.
July 26, 2018
Snakemake workflow for 10X Genomics whole genome sequencing data is now included in another Git repo. https://github.com/gavinha/TitanCNA10Xsnakemake
TitanCNA version 1.17.1 changes
1) New functions:
- correctIntegerCN(): recomputes high-level copy number that is capped by the maximum CN state.
Performs two tasks - (1) correct log ratio based on purity and ploidy, and then convert to decimal CN value; (2) Correct bins and segments in which the original predicted integer copy number was assigned the maximum CN state; bins and segments for all of chromosome X are also corrected, if provided in the input.
2) Modified functions:
- plotSegmentMedians() and plotCNlogRByChr(): includes argument to show color-coding for corrected copy number; defaults to TRUE for this argument.
3) Removed functions/manual/dependencies:
- extractAlleleReadCounts()
- Rsamtools dependency
TitanCNA version 1.15.0 changes
1) 10X Genomics analysis - Please see scripts for instructions on running the 10X Genomics analysis.
2) New script to help select optimal solutions.
Please see scripts/R_scripts
3) Added snakemake pipeline for entire TITAN workflow
Please see scripts/snakemake.
4) New function:
- plotSegmentMedians()
- loadHaplotypeAlleleCounts(): loads input allele counts with phasing information
- plotHaplotypeFraction(): results from 10X Genomics WGS data with phasing of haplotype blocks
5) Modified features (no changes for user-accessible functions): - updateParameters: coordinate descent estimate of ploidy update uses previously estimated normal parameter from the same corodinate descent iteration ; leads to faster convergence
Installation
Install TitanCNA R package from github
From within R-3.3.2 or higher,
install.packages("devtools")
library(devtools)
install_github("gavinha/TitanCNA")
Install TitanCNA from Bioconductor
From within R-3.3.2 or higher,
if (!requireNamespace("BiocManager", quietly=TRUE))
install.packages("BiocManager")
BiocManager::install("TitanCNA")
Install other dependencies
Install the HMMcopy suite Please follow instructions on the HMMcopy GitHub https://github.com/shahcompbio/hmmcopy_utils.
Install ichorCNA Please follow instructions on the ichorCNA GitHub Wiki https://github.com/broadinstitute/ichorCNA.
Usage
R scripts are provided to run the R component of the TITAN analysis using the TitanCNA R/Bioconductor package.
Please go to the scripts directory and look at the README there for more details.
Snakemake workflow
A snakemake is also provided in this repo.
This workflow will run the TITAN a set of tumour-normal pairs, starting from the BAM files and generating TitanCNA outputs. It will also perform model selection at the end of the workflow to choose the optimal ploidy and clonal cluster solutions.
Vignette in TitanCNA R package
The PDF of the vignette can be accessed from R
library(TitanCNA)
browseVignettes(package = "TitanCNA")
The path of the file can also be located using
pathToInstall <- system.file(package = "TitanCNA")
pathToPdf <- paste0(pathToInstall, "/int/doc/TitanCNA.pdf)
The example provided will reproduce Figure 1 in the manuscript. However, it will be slightly different because the example is only based on the analysis of chr2, not genome-wide.
Acknowledgements
TitanCNA was developed by Gavin Ha while in the laboratories of Sohrab Shah (sshah@bccrc.ca) and Sam Aparicio (saparicio@bccrc.ca) at the Dept of Molecular Oncology, BC Cancer Agency, Vancouver, Canada.
Yikan Wang and Daniel Lai have contributed code and discussions to this project.
The KRONOS TITAN workflow was developed by Diljot Grewal (dgrewal@bccrc.ca) and Jafar Taghiyar (jtaghiyar@bccrc.ca).
HMMcopy was co-developed by Daniel Lai and Gavin Ha.
TitanCNA was inspired by existing methods including OncoSNP and PyClone
Software License
License: GPLv3
TitanCNA R code is open source and R/Bioconductor package is under GPLv3. This applies to the v1.9.0 and all subsequent versions within and obtained from Bioconductor.
Users who are using TitanCNA earlier than v1.9.0 not for the purpose of academic research should contact gha@fredhutch.org, sshah@bccrc.ca, and prebstein@bccancer.bc.ca to inquire about previous licensing.
Owner
- Name: Gavin Ha
- Login: gavinha
- Kind: user
- Company: Fred Hutchinson Cancer Research Center
- Website: https://GavinHaLab.org
- Repositories: 2
- Profile: https://github.com/gavinha
GitHub Events
Total
- Issues event: 1
- Watch event: 3
- Fork event: 1
Last Year
- Issues event: 1
- Watch event: 3
- Fork event: 1
Committers
Last synced: about 1 year ago
Top Committers
| Name | Commits | |
|---|---|---|
| Gavin Ha | g****a@b****g | 174 |
| Gavin Ha | g****a@b****a | 38 |
| Luca Beltrame | l****e@m****t | 12 |
| Dan Tenenbaum | d****a@f****g | 10 |
| annahoge | 3****e | 6 |
| chapmanb | c****b@5****m | 5 |
| Bioconductor Git-SVN Bridge | b****c@b****g | 4 |
| Herve Pages | h****s@f****g | 4 |
| Dylan Hennessey | d****s@u****a | 2 |
| Hervé Pagès | h****s@f****g | 2 |
| Nitesh Turaga | n****a@g****m | 2 |
| vobencha | v****a@g****m | 2 |
| vobencha | v****n@r****g | 2 |
| LiNk-NY | m****9@g****m | 1 |
| Marc Carlson | m****n@f****g | 1 |
Committer Domains (Top 20 + Academic)
Issues and Pull Requests
Last synced: about 1 year ago
All Time
- Total issues: 82
- Total pull requests: 17
- Average time to close issues: 5 months
- Average time to close pull requests: 3 months
- Total issue authors: 47
- Total pull request authors: 10
- Average comments per issue: 2.32
- Average comments per pull request: 0.35
- Merged pull requests: 10
- Bot issues: 0
- Bot pull requests: 0
Past Year
- Issues: 0
- Pull requests: 0
- Average time to close issues: N/A
- Average time to close pull requests: N/A
- Issue authors: 0
- Pull request authors: 0
- Average comments per issue: 0
- Average comments per pull request: 0
- Merged pull requests: 0
- Bot issues: 0
- Bot pull requests: 0
Top Authors
Issue Authors
- lbeltrame (9)
- fpbarthel (8)
- aleighbrown (5)
- lounsburynw (5)
- ysbioinfo (4)
- gavinha (3)
- pchamely (3)
- lijinsgithub (2)
- thek71 (2)
- d-henness (2)
- elifirem (2)
- jgockley62 (2)
- lima1 (1)
- kieranrcampbell (1)
- ilykos (1)
Pull Request Authors
- chapmanb (6)
- annahoge (2)
- lbeltrame (2)
- aleighbrown (1)
- roryk (1)
- wayneliuq (1)
- d-henness (1)
- setgree (1)
- gaog94 (1)
- ZhaoxiangSimonCai (1)
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Packages
- Total packages: 1
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Total downloads:
- bioconductor 27,078 total
- Total dependent packages: 0
- Total dependent repositories: 0
- Total versions: 5
- Total maintainers: 1
bioconductor.org: TitanCNA
Subclonal copy number and LOH prediction from whole genome sequencing of tumours
- Homepage: https://github.com/gavinha/TitanCNA
- Documentation: https://bioconductor.org/packages/release/bioc/vignettes/TitanCNA/inst/doc/TitanCNA.pdf
- License: GPL-3
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Latest release: 1.45.0
published about 1 year ago
Rankings
Maintainers (1)
Dependencies
- R >= 3.5.1 depends
- GenomeInfoDb >= 1.8.7 imports
- GenomicRanges >= 1.24.3 imports
- IRanges >= 2.6.1 imports
- VariantAnnotation >= 1.18.7 imports
- data.table >= 1.10.4 imports
- dplyr >= 0.5.0 imports
- foreach >= 1.4.3 imports