dada2

Accurate sample inference from amplicon data with single nucleotide resolution

https://github.com/benjjneb/dada2

Science Score: 23.0%

This score indicates how likely this project is to be science-related based on various indicators:

  • CITATION.cff file
  • codemeta.json file
  • .zenodo.json file
  • DOI references
    Found 9 DOI reference(s) in README
  • Academic publication links
  • Committers with academic emails
    6 of 32 committers (18.8%) from academic institutions
  • Institutional organization owner
  • JOSS paper metadata
  • Scientific vocabulary similarity
    Low similarity (12.0%) to scientific vocabulary

Keywords

amplicon bioconductor bioinformatics metabarcoding metagenomics microbiome taxonomy

Keywords from Contributors

bioconductor-package gene genomics proteomics mass-spectrometry core-package u24ca289073 metabolomics human-cell-atlas single-cell-rna-seq
Last synced: 6 months ago · JSON representation

Repository

Accurate sample inference from amplicon data with single nucleotide resolution

Basic Info
Statistics
  • Stars: 516
  • Watchers: 38
  • Forks: 154
  • Open Issues: 167
  • Releases: 14
Topics
amplicon bioconductor bioinformatics metabarcoding metagenomics microbiome taxonomy
Created about 11 years ago · Last pushed 7 months ago
Metadata Files
Readme License

README.md

Build Status

dada2

Exact sample inference from high-throughput amplicon data. Resolves real variants differing by as little as one nucleotide. Visit the DADA2 website for the most detailed and up-to-date documentation.

Installation

The dada2 package binaries are available through Bioconductor:

```S

try http:// if https:// URLs are not supported

if (!requireNamespace("BiocManager", quietly=TRUE)) install.packages("BiocManager") BiocManager::install("dada2") ```

In order to install dada2 from source (and get the latest and greatest new features) see our installation from source instructions.

Documentation

The tutorial walkthrough of the DADA2 pipeline on paired end Illumina Miseq data.

The dada2 R package manual.

Further documentation is available on the DADA2 front page.

DADA2 Articles

DADA2: High resolution sample inference from Illumina amplicon data. Nature Methods, 2016. (Open Access link.)

Bioconductor workflow for microbiome data analysis: from raw reads to community analyses. F1000 Research, 2016.

Exact sequence variants should replace operational taxonomic units in marker-gene data analysis. ISMEJ, 2017.

High-throughput amplicon sequencing of the full-length 16S rRNA gene with single-nucleotide resolution. Nucleic Acids Research, 2019.

Other Resources

Planned feature improvements are publicly catalogued at the main DADA2 development site on github, specifically on the "Issues" page for DADA2:

https://github.com/benjjneb/dada2/issues

If the feature you are hoping for is not listed, you are welcome to add it as a feature request "issue" on this page. This request will be publicly available and listed on the page.

Bugs and difficulties in using DADA2 are also welcome on the issue tracker.

Owner

  • Name: Benjamin Callahan
  • Login: benjjneb
  • Kind: user
  • Location: Raleigh, NC
  • Company: NC State University

Physics -> Evolution -> Microbiome -> Bioinformatics

GitHub Events

Total
  • Issues event: 137
  • Watch event: 44
  • Issue comment event: 391
  • Push event: 13
  • Pull request event: 4
  • Pull request review event: 2
  • Fork event: 11
Last Year
  • Issues event: 137
  • Watch event: 44
  • Issue comment event: 391
  • Push event: 13
  • Pull request event: 4
  • Pull request review event: 2
  • Fork event: 11

Committers

Last synced: about 2 years ago

All Time
  • Total Commits: 706
  • Total Committers: 32
  • Avg Commits per committer: 22.063
  • Development Distribution Score (DDS): 0.382
Past Year
  • Commits: 6
  • Committers: 4
  • Avg Commits per committer: 1.5
  • Development Distribution Score (DDS): 0.667
Top Committers
Name Email Commits
benjjneb b****n@g****m 436
bcallah b****h@P****l 135
Gene Blanchard g****4@g****m 38
Nitesh Turaga n****a@g****m 16
Paul J. McMurdie j****1@g****m 14
Paul J. McMurdie m****e@a****u 14
Ben Callahan b****h@n****u 6
Fan Li f****b 4
Dan Tenenbaum d****a@f****g 4
Herve Pages h****s@f****g 4
J Wokaty j****y@s****u 4
Hervé Pagès h****s@f****g 3
Wojtek Bazant w****t@v****u 3
bcallah b****h@p****t 3
bcallah b****h@p****u 2
vobencha v****n@r****g 2
vobencha v****a@g****m 2
J Wokaty j****y 2
zachcp z****p@r****u 1
Dogrinev 6****v 1
Vladimir Mikryukov v****k 1
brendanf b****x@g****m 1
Michael McLaren m****2@g****m 1
Martin Morgan m****n@f****g 1
LiNk-NY m****9@g****m 1
Kayla-Morrell k****l@r****g 1
I-Hsuan Lin y****l@g****m 1
Florentin Constancias f****s@g****m 1
Fan Li f****b@g****m 1
Emanuel Heitlinger e****r@g****m 1
and 2 more...

Issues and Pull Requests

Last synced: 6 months ago

All Time
  • Total issues: 487
  • Total pull requests: 6
  • Average time to close issues: 11 months
  • Average time to close pull requests: 5 months
  • Total issue authors: 369
  • Total pull request authors: 5
  • Average comments per issue: 4.43
  • Average comments per pull request: 0.0
  • Merged pull requests: 2
  • Bot issues: 0
  • Bot pull requests: 0
Past Year
  • Issues: 122
  • Pull requests: 3
  • Average time to close issues: 20 days
  • Average time to close pull requests: 19 days
  • Issue authors: 99
  • Pull request authors: 2
  • Average comments per issue: 1.9
  • Average comments per pull request: 0.0
  • Merged pull requests: 0
  • Bot issues: 0
  • Bot pull requests: 0
Top Authors
Issue Authors
  • emankhalaf (10)
  • marwa38 (7)
  • benjjneb (7)
  • luigallucci (7)
  • andrebolerbarros (7)
  • Danyang1111 (6)
  • Ecotone23 (6)
  • hjarnek (5)
  • akspat (4)
  • lcamVz (4)
  • otaviolovison (4)
  • jspychalla (4)
  • Guillermouceda (4)
  • MadsBjornsen (4)
  • ep142 (4)
Pull Request Authors
  • ycl6 (2)
  • Micro-Biology (2)
  • cylyau (1)
  • leholman (1)
  • rabdill (1)
Top Labels
Issue Labels
enhancement (18) bug (11) Priority (8) documentation (8) tutorial (4) question (4) support (2) help wanted (2)
Pull Request Labels

Packages

  • Total packages: 1
  • Total downloads:
    • bioconductor 795,009 total
  • Total dependent packages: 2
  • Total dependent repositories: 0
  • Total versions: 5
  • Total maintainers: 1
bioconductor.org: dada2

Accurate, high-resolution sample inference from amplicon sequencing data

  • Versions: 5
  • Dependent Packages: 2
  • Dependent Repositories: 0
  • Downloads: 795,009 Total
Rankings
Dependent repos count: 0.0%
Stargazers count: 0.0%
Forks count: 0.1%
Downloads: 3.1%
Average: 3.5%
Dependent packages count: 14.4%
Last synced: 6 months ago

Dependencies

DESCRIPTION cran
  • R >= 3.4.0 depends
  • Rcpp >= 0.12.0 depends
  • methods >= 3.4.0 depends
  • BiocGenerics >= 0.22.0 imports
  • Biostrings >= 2.42.1 imports
  • IRanges >= 2.6.0 imports
  • RcppParallel >= 4.3.0 imports
  • ShortRead >= 1.32.0 imports
  • XVector >= 0.16.0 imports
  • ggplot2 >= 2.1.0 imports
  • parallel >= 3.2.0 imports
  • reshape2 >= 1.4.1 imports
  • BiocStyle * suggests
  • knitr * suggests
  • rmarkdown * suggests