ImmuneSpaceR

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Please update the URL (machine name) to datatools.immunespace.org from www.immunespace.org in your .netrc file.

A thin wrapper around Rlabkey to access the ImmuneSpace Data & Tools Portal from R.

This package simplifies access to the HIPC ImmuneSpace database for R programmers. It takes advantage of the standardization of the database to hide all the Rlabkey specific code away from the user. The study-specific datasets can be accessed via an object-oriented paradigm.

Installation

Install from Bioconductor:

r install.packages("BiocManager") BiocManager::install("ImmuneSpaceR")

Or install the latest development version via remotes:

r install.packages("remotes") remotes::install_github("RGLab/ImmuneSpaceR")

Configuration

The ImmuneSpace Portal can be accessed via ImmuneSpaceR with the user's credentials. A .netrc file storing login and password information is required.

1. Register
2. Create a netrc file with your ImmuneSpace credetntials using interactive_netrc() function in R:

r library(ImmuneSpaceR) interactive_netrc()

If you're familiar with the command-line interface, see the introductory vignette.

Usage

Create a connection

The general idea is that the user creates an instance of an ImmuneSpaceConnection class. The instance configures itself to connect to a specific study, and datasets and gene expression matrices can be retrieved by name.

For example:

r library(ImmuneSpaceR) con <- CreateConnection("SDY269")

will create an instance of SDY269.

List datasets

Datasets can be listed by:

r con$listDatasets()

which will print names of available datasets and gene expression matrices.

Retrieve datasets

Gene expression matrices or datasets can be retreived by:

r LAIV2008 <- con$getGEMatrix("SDY269_PBMC_LAIV_Geo") elisa <- con$getDataset("elisa")

The connection object caches data, so once it is retrieved, the next time you access it, it will use the local cached copy. The package uses a R6 class system to represent the connection to a study and get around some of R's copy-on-change behaviour.

Visualize

The plot method uses ggplot2 functions to generate visualizations of datasets, leveraging the standardized dataset tables.

r con$plot("hai")

Examples & Documentation

For more advanced examples and detailed documentation, see the package vignettes and the reports available on ImmuneSpace.

Contributing

If you'd like to report bugs/issues/feature requests or contribute to the package, please see the contributing guidelines and join our Slack workspace.